Human Troponin T type 1 (slow skeletal) ELISA Kit (Colorimetric)

ELISA: Human Troponin T type 1 (slow skeletal) ELISA Kit (Colorimetric) [NBP2-76601] - Samples were spiked with high concentrations of Human Troponin T type 1 (slow skeletal) and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Troponin T type 1 (slow skeletal) ELISA Kit (Colorimetric) [NBP2-76601] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.625 - 1000pg/mL
• Sensitivity: 9.375pg/mL

Human Troponin T type 1 (slow skeletal) ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human TNNT1 in samples. No significant cross-reactivity or interference between Human TNNT1 and analogues was observed.
• Standard Curve Range: 15.625 - 1000pg/mL
• Sensitivity: 9.375pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.88
• Intra-Assay: CV% < 4.97
• Spike Recovery: 89-109%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TNNT1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Troponin T type 1 (slow skeletal) ELISA Kit (Colorimetric)

• ANMtroponin T, slow skeletal muscle
• FLJ98147
• MGC104241
• Slow skeletal muscle troponin T
• STNT
• TnTs
• TNTtroponin T1, skeletal, slow
• troponin T type 1 (skeletal, slow)
• troponin-T1, skeletal, slow

Background

Troponin T type 1 encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Troponin T type 1 (slow skeletal) ELISA Kit (NBP2-76601). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TNNT1