Troponin T type 1 (slow skeletal) Antibody

Western Blot: Troponin T type 1 (slow skeletal) Antibody [NBP2-26095] - Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Troponin T type 1 (slow skeletal) Antibody [NBP2-26095] - Staining of paraffin embedded Human Skeletal Muscle. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Po, Bv, Ca
• Applications: WB, ELISA, IHC, Single-Cell Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Troponin T type 1 (slow skeletal) Antibody Summary

• Description: Novus Biologicals Goat Troponin T type 1 (slow skeletal) Antibody (NBP2-26095) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Troponin T type 1 (slow skeletal) Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EKAQELSDWIHQLE corresponding to internal region according to NP_003274.3, NP_001119604.1, NP_001119605.1.
• Specificity: This antibody is expected to recognize all reported isoforms (NP_003274.3; NP_001119604.1; NP_001119605.1).
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNNT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Single Cell Western 100 ug/mL
  • Western Blot 0.03 - 0.1 ug/mL
• Application Notes: WB: Approx. 37+35 kDa band bands observed in human skeletal muscle lysates (calculated MW of 32.9 kDa band according to NP_003274.3). This molecular weight is routinely observed by other sources. SCW validated using iPSC-derived cardiomyocytes.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Troponin T type 1 (slow skeletal) Antibody

• ANMtroponin T, slow skeletal muscle
• FLJ98147
• MGC104241
• Slow skeletal muscle troponin T
• STNT
• TnTs
• TNTtroponin T1, skeletal, slow
• troponin T type 1 (skeletal, slow)
• troponin-T1, skeletal, slow

Background

Troponin T type 1 encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Troponin T type 1 (slow skeletal) Antibody (NBP2-26095)(1)

Publication using NBP2-26095

• Midde K, Dumka V, Pinto JR et al. Myosin cross-bridges do not form precise rigor bonds in hypertrophic heart muscle carrying troponin T mutations. J Mol Cell Cardiol 2011-09-01 [PMID: 21683708]

Bioinformatics

• Gene Symbol: TNNT1
• Entrez:
  • 7138(Human)