Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free

Western Blot: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Western blot analysis using TNNI2 mAb against HEK293 (1) and TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Immunofluorescence analysis of Hela cells using TNNI2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow Cytometry: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Flow cytometric analysis of NIH/3T3 cells using TNNI2 mouse mAb (green) and negative control (red).

Western Blot: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Western blot analysis using TNNI2 mAb against human TNNI2 (AA: 1-182) recombinant protein. (Expected MW is 21 kDa)

ELISA: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, CyTOF-ready
• Clone: 2F12G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free Summary

• Description: Novus Biologicals Mouse Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free (NBP2-37536) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Troponin I type 2 (fast skeletal) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNNI2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free

• skeletal, fast
• troponin I type 2 (skeletal, fast)
• troponin I, fast skeletal muscle
• Troponin I, fast-twitch isoform
• troponin I, fast-twitch skeletal muscle isoform

Background

The troponin I type 2 gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNNI2