Troponin I Type 1 (slow skeletal) Antibody

Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP2-26202] - Staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Po, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Troponin I Type 1 (slow skeletal) Antibody Summary

• Description: Novus Biologicals Goat Troponin I Type 1 (slow skeletal) Antibody (NBP2-26202) is a polyclonal antibody validated for use in WB and ELISA. Anti-Troponin I Type 1 (slow skeletal) Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence PEVERKPKITASRK-C corresponding to N-Terminus according to NP_003272.3.
• Predicted Species: Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNNI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 25 kDa band observed in human and mouse skeletal muscle lysates (calculated MW of 21.7 kDa band according to NP_003272.3).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Troponin I Type 1 (slow skeletal) Antibody

• DKFZp451O223
• slow-twitch isoform
• SSTNI
• TNN1
• troponin I type 1 (skeletal, slow)
• troponin I, skeletal, slow
• troponin I, slow skeletal muscle

Background

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Troponin I Type 1 (slow skeletal) Antibody (NBP2-26202)(1)

Publication using NBP2-26202

• Engel PL, Kobayashi T, Biesiadecki B et al. Identification of a region of troponin I important in signaling cross-bridge-dependent activation of cardiac myofilaments. J Biol Chem 2007-01-05 [PMID: 17099250]

Bioinformatics

• Gene Symbol: TNNI1
• Entrez:
  • 7135(Rat)
  • 7135(Rat)