TRNT1 Antibody (1G11) - Azide and BSA Free

Western Blot: TRNT1 Antibody (1G11) [H00051095-M01] - Analysis of TRNT1 expression in transfected 293T cell line by TRNT1 monoclonal antibody (M01), clone 1G11.Lane 1: TRNT1 transfected lysate (Predicted MW: 46.4 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: TRNT1 Antibody (1G11) [H00051095-M01] - Detection limit for recombinant GST tagged TRNT1 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TRNT1 Antibody (1G11) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TRNT1 Antibody (1G11) - Azide and BSA Free (H00051095-M01) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: TRNT1 (AAH12537, 1 a.a. ~ 405 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKLQSPEFQSLFTEGLKSLTELFVKENHELRIAGGAVRDLLNGVKPQDIDFATTATPTQMKEMFQSAGIRMINNRGEKHGTITARLHEENFEITTLRIDVTTDGRHAEVEFTTDWQKDAERRDLTINSMFLGFDGTLFDYFNGYEDLKNKKVRFVGHAKQRIQEDYLRILRYFRFYGRIVDKPGDHDPETLEAIAENAKGLAGISGERIWVELKKILVGNHVNHLIHLIYDLDVAPYIGLPANASLEEFDKVSKNVDGFSPKPVTLLASLFKVQDDVTKLDLRLKIAKEEKNLGLFIVKNRKDLIKATDSSDPLKPYQDFIIDSREPDATTRVCELLKYQGEHCLLKEMQQWSIPPFPVSGHDIRKVGISSGKEIGALLQQLREQWKKSGYQMEKDELLSYIKKT
• Specificity: TRNT1 - tRNA nucleotidyl transferase, CCA-adding, 1 (1G11)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRNT1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TRNT1 Antibody (1G11) - Azide and BSA Free

• CCA1
• CCA-adding
• CGI-47
• EC 2.7.7.72
• mitochondrial CCA-adding tRNA-nucleotidyltransferase
• mt CCA-adding enzyme
• mt tRNA adenylyltransferase
• mt tRNA CCA-diphosphorylase
• mt tRNA CCA-pyrophosphorylase
• MtCCA
• tRNA nucleotidyl transferase, CCA-adding, 1
• tRNA-nucleotidyltransferase 1, mitochondrial

Background

The CCA-adding enzyme TRNT1 (EC 2.7.7.25) is an essential enzyme that catalyzes the addition of the CCA terminus to the 3-prime end of tRNA precursors. This reaction is a fundamental prerequisite for mature tRNAs to become aminoacylated and to participate in protein biosynthesis (Lizano et al., 2007 (PubMed 17204286)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRNT1
• Entrez:
  • 51095(Human)
• Uniprot:
  • AAH12537(Human)