TRIM32 Antibody [Alexa Fluor® 700]

• Reactivity: Hu, Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

TRIM32 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human TRIM32
  Arg105-Lys204
  Accession # Q13049
• Specificity: Detects human and mouse TRIM32 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRIM32 Antibody [Alexa Fluor® 700]

• 72 kDa Tat-interacting protein
• BBS11
• BBS1172-KD
• EC 6.3.2.-
• HT2A
• HT2Alimb girdle muscular dystrophy 2H (autosomal recessive)
• LGMD2H
• TATIP
• TRIM32
• tripartite motif containing 32
• Zinc finger protein HT2A
• zinc-finger protein HT2A

Background

TRIM32 (Tripartite motif-containing protein 32; also 72 kDa TAT-interacting protein, and zinc finger protein HT2A) is a 72-82 kDa member of the TRIM/RBCC family of proteins. It is an E3 ligase that is found in structures termed nuclear and cytoplasmic bodies. Cells known to express TRIM32 are diverse and include fibroblasts, keratinocytes, skeletal muscle cells and neurons. TRIM32 ubiquitinates select proteins such as c-myc, Abi2, actin and dysbindin. Human TRIM32 is 653 amino acids (aa) in length. It contains one E3 ligase RING finger domain (aa 20-65), a B-Box type zinc-finger region (aa 103-133), a coiled-coil region (aa 138-197), five NHL repeats (aa 358-646) and three utilized phosphorylation sites (Ser328/335/339). TRIM32 has the potential to form homomultimers. Over aa 105-204, human TRIM32 exhibits 95% aa identity with mouse TRIM32.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.