Trim22 Overexpression Lysate

Western Blot: Trim22 Overexpression Lysate (Adult Normal) [NBL1-17282] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Trim22.

• Reactivity: Hu
• Applications: WB

Trim22 Overexpression Lysate Summary

• Description:

  Trim22 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC207431
  
  Accession#: NM_006074
  
  Protein Tag: C-MYC/DDK
• Gene: TRIM22

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 56.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Trim22 Overexpression Lysate

• 50 kDa-stimulated trans-acting factor
• E3 ubiquitin-protein ligase TRIM22
• EC 6.3.2
• EC 6.3.2.-
• GPSTAF50
• RING finger protein 94
• RNF94stimulated trans-acting factor (50 kDa)
• Staf-50
• STAF50staf-50
• tripartite binding motif 22
• tripartite motif containing 22
• tripartite motif protein TRIM22
• tripartite motif-containing 22
• Tripartite motif-containing protein 22

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the cytoplasm and its expression is induced by interferon. The protein down-regulates transcription from the HIV-1 LTR promoter region, suggesting that function of this protein may be to mediate interferon's antiviral effects. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: TRIM22