Trim22 Overexpression Lysate

Images

 
Western Blot: Trim22 Overexpression Lysate (Adult Normal) [NBL1-17282] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Trim22.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Trim22 Overexpression Lysate Summary

Description

Trim22 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC207431

Accession#: NM_006074

Protein Tag: C-MYC/DDK
Gene
TRIM22

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
56.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Trim22 Overexpression Lysate

  • 50 kDa-stimulated trans-acting factor
  • E3 ubiquitin-protein ligase TRIM22
  • EC 6.3.2
  • EC 6.3.2.-
  • GPSTAF50
  • RING finger protein 94
  • RNF94stimulated trans-acting factor (50 kDa)
  • Staf-50
  • STAF50staf-50
  • tripartite binding motif 22
  • tripartite motif containing 22
  • tripartite motif protein TRIM22
  • tripartite motif-containing 22
  • Tripartite motif-containing protein 22

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the cytoplasm and its expression is induced by interferon. The protein down-regulates transcription from the HIV-1 LTR promoter region, suggesting that function of this protein may be to mediate interferon's antiviral effects. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Trim22 Lysate (NBL1-17282) (0)

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Bioinformatics

Gene Symbol TRIM22