Trappin-2/Elafin/Skalp Overexpression Lysate (Native)

Images

 
Western Blot: Skalp Overexpression Lysate (Adult Normal) [NBL1-14379] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Skalp.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Trappin-2/Elafin/Skalp Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203136 and based on accession number NM_002638. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens peptidase inhibitor 3, skin-derived (PI3), mRNA.
Gene
PI3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
9.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Trappin-2/Elafin/Skalp

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Trappin-2/Elafin/Skalp Overexpression Lysate (Native)

  • cementoin
  • Elafin
  • Elastase-specific inhibitor
  • ESI
  • ESIWAP four-disulfide core domain protein 14
  • Peptidase inhibitor 3
  • peptidase inhibitor 3, skin-derived
  • PI3
  • PI-3
  • pre-elafin
  • protease inhibitor 3, skin-derived (SKALP)
  • Protease inhibitor WAP3
  • SKALP
  • SKALPELAFIN
  • Skin-derived antileukoproteinase
  • Trappin2
  • Trappin-2
  • WAP four-disulfide core domain 14
  • WAP3MGC13613
  • WFDC14elafin

Background

This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Trappin-2/Elafin/Skalp Lysate (NBL1-14379) (0)

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Bioinformatics

Gene Symbol PI3
Entrez