Trappin-2/Elafin/Skalp Antibody (257729) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 257729
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Trappin-2/Elafin/Skalp Antibody (257729) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Trappin‑2/Elafin
  Ala23-Gln117
  Accession # P19957
• Specificity: Detects the full length form (amino acids 23‑117) and the trypsin-processed form (amino acids 61‑117) of human Trappin‑2/Elafin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human SLPI is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PI3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Trappin-2/Elafin/Skalp Antibody (257729) [Unconjugated]

• cementoin
• Elafin
• Elastase-specific inhibitor
• ESI
• ESIWAP four-disulfide core domain protein 14
• Peptidase inhibitor 3
• peptidase inhibitor 3, skin-derived
• PI3
• PI-3
• pre-elafin
• protease inhibitor 3, skin-derived (SKALP)
• Protease inhibitor WAP3
• SKALP
• SKALPELAFIN
• Skin-derived antileukoproteinase
• Trappin2
• Trappin-2
• WAP four-disulfide core domain 14
• WAP3MGC13613
• WFDC14elafin

Background

Trappin-2 is the human member of the trappin gene family that contains SLPI (1). Trappin-2 consists of an N-terminal transglutaminase substrate domain (residues
23‑60) and a C-terminal four-disulfide core or whey acidic protein (WAP) domain (residues 72‑117). Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leucoproteinase) are alternative names for Trappin-2 and reflect its protease targets. However, elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule, which can be isolated naturally. The recombinant human Trappin-2 corresponds to the full-length form (residues 23‑117), which migrates as two protein bands under SDS-PAGE due to an unidentified mechanism. In addition to its ability to inhibit human neutrophil elastase, it can also be used as a substrate for transglutaminases.

1. Schalkwijk, J. et al. (1999) Biochem. J. 340:569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Trappin-2/Elafin/Skalp Antibody (MAB1747)(2)

Publications using MAB1747

• Tantengco OAG, Kechichian T, Vincent KL Et al. Inflammatory Response Elicited by Ureaplasma parvum colonization in human cervical epithelial, stromal, and immune cells Reproduction (Cambridge, England) 11/1/2021 [PMID: 34780348]
• Viraj J. Jasinghe, Erika Arnau Peyrotte, Adrienne F.A. Meyers, Niranjala Gajanayaka, Terry B. Ball, Paul Sandstrom et al. Human rElafin Inhibits HIV-1 Replication in Its Natural Target Cells BioResearch Open Access 2013-04-18 [PMID: 23593565]

Bioinformatics

• Gene Symbol: PI3
• Uniprot:
  • P19957()