Transglutaminase 3/TGM3 Antibody

Immunocytochemistry/Immunofluorescence: Transglutaminase 3/TGM3 Antibody [NBP2-94466] - Analysis of U2OS cells using Transglutaminase 3/TGM3 . Blue: DAPI for nuclear staining.

Western Blot: Transglutaminase 3/TGM3 Antibody [NBP2-94466] - Analysis of A-549 using Transglutaminase 3/TGM3 at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 0.5s.

Product Discontinued

Transglutaminase 3/TGM3 Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TGM3 (NP_003236.3).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGM3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:100
  • Western Blot 1:100 - 1:500
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for Transglutaminase 3/TGM3 Antibody

• EC 2.3.2.13
• MGC126250
• protein-glutamine gamma-glutamyltransferase E
• TG(E)
• TG3
• TGase E
• TGase-3
• TgaseE
• TGEMGC126249
• TGM3
• transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Transglutaminase 3
• Transglutaminase E
• transglutaminase-3

Background

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium dependent. Transglutaminase 3 consists of two polypeptide chains activated from a single precursor protein by proteolysis. Transglutaminase 3 is involved the later stages of cell envelope formation in the epidermis and hair follicle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TGM3