Transgelin/TAGLN/SM22 alpha Antibody

Images

 
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transgelin protein stained by Transgelin antibody ...read more
Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Coronary artery vascular smooth muscle cells in fixed, frozen adult mouse heart. Primary antibody 1:100 dilution of a 0.2mg/ml solution. This ...read more
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin antibody diluted by 1:1000.
Biological Strategies: Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Western blot analysis of Transgelin and beta-actin (lower panel) Sample (35 ug of whole cell lysate) A: A-10 cell B: A-10 ...read more
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Paraffin-embedded mouse heart. Transgelin antibody dilution: 1:500.
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Paraffin-embedded mouse esophagus. Transgelin antibody dilution: 1:500.
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - 40X image of coronary artery vascular smooth muscle stained for transgelin. Primary Antibody dilution of 1:100. This image was submitted ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PoSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

Transgelin/TAGLN/SM22 alpha Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Transgelin. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Porcine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TAGLN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 3
using
NBP2-20405 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (87%), Xenopus laevis (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Transgelin/TAGLN/SM22 alpha Antibody

  • DKFZp686P11128
  • Protein WS3-10
  • SM22 alpha
  • SM2222 kDa actin-binding protein
  • SMCCDKFZp686B01212
  • Smooth muscle protein 22-alpha
  • TAGLN
  • TAGLN1
  • transgelin variant 2
  • Transgelin
  • WS3-10
  • WS3-10SM22-alpha

Background

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Transgelin/TAGLN/SM22 alpha Antibody (NBP2-20405) (0)

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Reviews for Transgelin/TAGLN/SM22 alpha Antibody (NBP2-20405) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NBP2-20405:
Filter by Applications
IHC
(2)
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Filter by Species
Mouse
(2)
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Images Ratings Applications Species Date Details
Immunohistochemistry Transgelin/TAGLN/SM22 alpha NBP2-20405
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reviewed by:
Anonymous
IHC Mouse 06/08/2017
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Summary

ApplicationImmunohistochemistry
Sample TestedAdult heart
SpeciesMouse
LotCGVL0115111

Comments

CommentsSample was fixed overnight in 4% PFA, blocked with 1% BSA; 1.5% Normal donkey serum; 0.1% triton X for 1 hour, ON with primary antibody 1:100 dilution of a 0.2mg/ml solution, washed, stained 1 hour with gt antisheep antibody 1:500 concentration.
Immunohistochemistry Transgelin/TAGLN/SM22 alpha NBP2-20405
Enlarge
reviewed by:
Michelle Tallquist
IHC Mouse 05/03/2017
View

Summary

ApplicationImmunohistochemistry
Sample TestedAdult heart
SpeciesMouse
Lot41815

Comments

CommentsFixed o.n. 4% PFA, block 1 hour 1%BSA in 0.1% Triton/PBS, 1:100 dilution antibody in 0.1% Triton/PBS o.n., 1 hour secondary.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Transgelin/TAGLN/SM22 alpha Antibody (NBP2-20405) (0)

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Recent Reviews

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Anonymous
06/08/2017
Application: IHC
Species: Mouse

Michelle Tallquist
05/03/2017
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol TAGLN
Uniprot