Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transgelin protein stained by Transgelin antibody ...read more
Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Coronary artery vascular smooth muscle cells in fixed, frozen adult mouse heart. Primary antibody 1:100 dilution of a 0.2mg/ml solution. This ...read more
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin antibody diluted by 1:1000.
Biological Strategies: Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Western blot analysis of Transgelin and beta-actin (lower panel) Sample (35 ug of whole cell lysate) A: A-10 cell B: A-10 ...read more
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - 40X image of coronary artery vascular smooth muscle stained for transgelin. Primary Antibody dilution of 1:100. This image was submitted ...read more
23 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 2 Reviews rated 3 using NBP2-20405 in the following applications:
Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (87%), Xenopus laevis (81%).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7), 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for Transgelin/TAGLN/SM22 alpha Antibody
SM2222 kDa actin-binding protein
Smooth muscle protein 22-alpha
transgelin variant 2
The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Sample was fixed overnight in 4% PFA, blocked with 1% BSA; 1.5% Normal donkey serum; 0.1% triton X for 1 hour, ON with primary antibody 1:100 dilution of a 0.2mg/ml solution, washed, stained 1 hour with gt antisheep antibody 1:500 concentration.
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