Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8)

Western Blot: SM22 alpha Antibody (8C8) [NBP2-00894] Analysis of extracts (35ug) from 9 different cell lines by using anti-SM22 alpha monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) [NBP2-00894] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded human breast cancer tissue using anti-TAGLN mouse monoclonal antibody. (Heat-induced epitope retrieval, Data courtesy of a collaborative pathologist.

Western Blot: SM22 alpha Antibody (8C8) [NBP2-00894] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SM22 alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SM22 alpha.

Immunohistochemistry-Paraffin: SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SM22 alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SM22 alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SM22 alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SM22 alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded Human breast tissue using anti-SM22 alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded Human endometrium tissue using anti-SM22 alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded Human prostate tissue using anti-SM22 alpha mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI8C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) Summary

• Immunogen: Full length human reocombinant protein of human TAGLN(NP_003177) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TAGLN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 22.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8)

• DKFZp686P11128
• Protein WS3-10
• SM22 alpha
• SM2222 kDa actin-binding protein
• SMCCDKFZp686B01212
• Smooth muscle protein 22-alpha
• TAGLN
• TAGLN1
• transgelin variant 2
• Transgelin
• WS3-10
• WS3-10SM22-alpha

Background

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TAGLN
• Entrez:
  • 6876(Human)