Transcription factor E3 Antibody

Images

 
Western Blot: Transcription factor E3 Antibody [NBP1-89976] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Transcription factor E3 Antibody [NBP1-89976] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody [NBP1-89976] - Immunohistochemical staining of human kidney shows moderate nuclear positivity in renal glomeruli and tubuli cells.
Western Blot: Transcription factor E3 Antibody [NBP1-89976] - Analysis in human cell line U-2 OS.
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody [NBP1-89976] - Immunohistochemical staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody [NBP1-89976] - Immunohistochemical staining of human prostate shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody [NBP1-89976] - Immunohistochemical staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
Simple Western: Transcription factor E3 Antibody [NBP1-89976] - Simple Western lane view shows a specific band for TFE3 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the ...read more
Simple Western: Transcription factor E3 Antibody [NBP1-89976] - Electropherogram image(s) of corresponding Simple Western lane view. Transcription factor E3 antibody was used at 1:30 dilution on RT-4 lysate(s).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Transcription factor E3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:SKDLESRQRSLEQANRSLQLRIQELELQAQIHGLPVPPTPGLLSLATTSASDSLKPEQLDIEEEGRPGAATFHVGGGPAQNAPHQQPPAPPSDALLDLHFPSDHLGDLGDPFHLGLEDILMEEEEGVVGGLSGGALSPLRAAS
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TFE3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Simple Western 1:30
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
Transcription factor E3 Protein (NBP1-89976PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-89976 in the following applications:

Publications
Read Publications using
NBP1-89976 in the following applications:

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23582324)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Transcription factor E3 Antibody

  • BHLHE33
  • bHLHe33transcription factor E3
  • Class E basic helix-loop-helix protein 33
  • RCCP2
  • TFEA
  • transcription factor binding to IGHM enhancer 3
  • transcription factor E family, member A
  • Transcription factor for IgH enhancer

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-89976
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for Transcription factor E3 Antibody (NBP1-89976)(5)

Review for Transcription factor E3 Antibody (NBP1-89976) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Pig.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-89976:
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ICC
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Transcription factor E3 NBP1-89976
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reviewed by:
Anonymous
ICC Pig 02/09/2017
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Summary

ApplicationImmunocytochemistry
Sample Testedprimary pig RPE
SpeciesPig
LotE106019

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Transcription factor E3 Antibody (NBP1-89976) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Anonymous
02/09/2017
Application: ICC
Species: Pig

Bioinformatics

Gene Symbol TFE3