Transcription factor E3 Antibody (CL12810) - Azide and BSA Free

Staining of human kidney shows moderate to strong nuclear positivity in cells in glomeruli.

Staining of human kidney cancer shows strong nuclear positivity in tumor cells.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human ovarian cancer (clear cell carcinoma) shows moderate nuclear positivity in a subset of tumor cells.

Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.

Analysis in human cell line U-2 OS.

Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL12810
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Transcription factor E3 Antibody (CL12810) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Transcription factor E3 Antibody (CL12810) - Azide and BSA Free (NBP3-18574) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P19532, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TFE3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Transcription factor E3 Antibody (CL12810) - Azide and BSA Free

• BHLHE33
• bHLHe33transcription factor E3
• Class E basic helix-loop-helix protein 33
• RCCP2
• TFEA
• transcription factor binding to IGHM enhancer 3
• transcription factor E family, member A
• Transcription factor for IgH enhancer

Background

TFE3, a member of the helix-loop-helix family of transcription factors, binds to the mu-E3 motif of the immunoglobulin heavy-chain enhancer and is expressed in many cell types (Henthorn et al., 1991 [PubMed 1685140]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TFE3