TRAILR4/TNFRSF10D/DcR2 Antibody (104918) [Phycoerythrin]

Human peripheral blood granulocytes were stained with Mouse Anti-Human TRAIL R4/TNFRSF10D PE-conjugated Monoclonal Antibody (Catalog # FAB633P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 104918
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

TRAILR4/TNFRSF10D/DcR2 Antibody (104918) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TRAIL R4/TNFRSF10D
  Ala56-His211
  Accession # Q9UBN6
• Specificity: Detects human TRAIL R4/TNFRSF10D in ELISAs and Western blots. In sandwich immunoassays, less than 5% cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, TRAIL R3, rhTRAIL, rhTNF‑ alpha , and rhTNF-beta is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF10D
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAILR4/TNFRSF10D/DcR2 Antibody (104918) [Phycoerythrin]

• CD264 antigen
• CD264
• DcR2
• DCR2decoy with truncated death domain
• Decoy receptor 2
• TNF receptor-related receptor for TRAIL
• TNF-related apoptosis-inducing ligand receptor 4
• TNFRSF10D
• TRAIL R4
• TRAILR4
• TRAIL-R4
• TRAILR4TRAIL receptor 4
• TRUNDDTRAIL receptor with a truncated death domain
• tumor necrosis factor receptor superfamily member 10D
• tumor necrosis factor receptor superfamily, member 10d, decoy with truncateddeath domain

Background

Human TRAIL R4, also called Decoy Receptor 2 (DcR2) and TRUNND (TRAIL Receptor with a Truncated Death Domain), is a type I, TNF R family transmembrane protein, which is a receptor for TRAIL (APO2 Ligand). In the TNF superfamily nomenclature, TRAIL R4 is designated as TNFRSF10D. TRAIL R4 is unique among the TRAIL receptors in that its cytoplasmic domain contains a truncated consensus death domain motif. Binding of TRAIL R4 does not result in an apoptotic signal. Overexpression of TRAIL R4 can protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-mediated apoptosis. The human soluble TRAIL R4/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

1. Griffith, T.S. et al. (1998) Curr. Opin. Immunol. 10:559.
2. Pan, G. et al. (1998) FEBS lett 424:41.
3. Marsters, S.A. et al. (1997) Cur. Biol. 7:1003.
4. Degli-Esposti, M.A. et al. (1997) Immunity 7:813.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAILR4/TNFRSF10D/DcR2 Antibody (FAB633P)(18)

Publications using FAB633P

• JD Greenlee, M Lopez-Cave, N Ortiz-Oter, K Liu, T Subramania, B Cagir, MR King Oxaliplatin resistance in colorectal cancer enhances TRAIL sensitivity via death receptor 4 upregulation and lipid raft localization Elife, 2021-08-03;10(0):. 2021-08-03 [PMID: 34342264] (Flow Cytometry, Human)
• Yu Ren, Xue Wang, Shuaishuai Huang, Yangkai Xu, Guobin Weng, Rui Yu Alternol Sensitizes Renal Carcinoma Cells to TRAIL-Induced Apoptosis Frontiers in Pharmacology 2021-03-25 [PMID: 33841136]
• Madsen SD, Jones SH, Tucker HA et al. Survival of aging CD264+ and CD264- populations of human bone marrow mesenchymal stem cells is independent of colony-forming efficiency Biotechnol. Bioeng. 10/15/2019 [PMID: 31612990]
• EB Jo, YS Lee, H Lee, JB Park, H Park, YL Choi, D Hong, SJ Kim Combination therapy with c-met inhibitor and TRAIL enhances apoptosis in dedifferentiated liposarcoma patient-derived cells BMC Cancer, 2019-05-24;19(1):496. 2019-05-24 [PMID: 31126284] (Flow Cytometry, Human)
• SD Madsen, KC Russell, HA Tucker, J Glowacki, BA Bunnell, KC O'Connor Decoy TRAIL receptor CD264: a cell surface marker of cellular aging for human bone marrow-derived mesenchymal stem cells Stem Cell Res Ther, 2017-09-29;8(1):201. 2017-09-29 [PMID: 28962588] (Flow Cytometry, Human)
• D Jaworska, E Szliszka Targeting Apoptotic Activity Against Prostate Cancer Stem Cells Int J Mol Sci, 2017-07-29;18(8):. 2017-07-29 [PMID: 28758908] (Flow Cytometry, Human)
• HD Singh, I Otano, K Rombouts, KP Singh, D Peppa, US Gill, K Böttcher, PTF Kennedy, J Oben, M Pinzani, H Walczak, G Fusai, WMC Rosenberg, MK Maini TRAIL regulatory receptors constrain human hepatic stellate cell apoptosis Sci Rep, 2017-07-17;7(1):5514. 2017-07-17 [PMID: 28717244] (Flow Cytometry, Human)
• Tang X, Lu J, Tu H, Huang K, Fu R, Cao G, Huang M, Cheng L, Dai L, Zhang L TRAIL-engineered bone marrow-derived mesenchymal stem cells: TRAIL expression and cytotoxic effects on C6 glioma cells. Anticancer Res, 2014-02-01;34(2):729-34. 2014-02-01 [PMID: 24511006] (Flow Cytometry, Rat)
• J Trebing, M El-Mesery, V Schäfer, D Weisenberger, D Siegmund, K Silence et al. CD70-restricted specific activation of TRAILR1 or TRAILR2 using scFv-targeted TRAIL mutants Cell Death & Disease 2014-01-30 [PMID: 24481449]
• Kim , Seong Mu, Woo , Ji Sun, Jeong , Chang Hy, Ryu , Chung He, Jang , Jae-Deog, Jeun , Sin-Soo Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma. Stem Cells Transl Med, 2014-01-16;3(2):172-82. 2014-01-16 [PMID: 24436439] (Flow Cytometry, Human)
• Catherine J. Walsh, Carl A. Luer, Jennifer E. Yordy, Theresa Cantu, Jodi Miedema, Stephanie R. Leggett et al. Epigonal Conditioned Media from Bonnethead Shark, Sphyrna tiburo, Induces Apoptosis in a T-Cell Leukemia Cell Line, Jurkat E6-1 Marine Drugs 2013-08-26 [PMID: 24065163]
• Gojo I, Tan M, Fang H, Sadowska M, Lapidus R, Baer M, Carrier F, Beumer J, Anyang B, Srivastava R, Espinoza-Delgado I, Ross D Translational phase I trial of vorinostat (suberoylanilide hydroxamic acid) combined with cytarabine and etoposide in patients with relapsed, refractory, or high-risk acute myeloid leukemia. Clin Cancer Res, 2013-02-12;19(7):1838-51. 2013-02-12 [PMID: 23403629] (Flow Cytometry, Human)
• Kalb ML, Glaser A, Stary G, Koszik F, Stingl G TRAIL(+) human plasmacytoid dendritic cells kill tumor cells in vitro: mechanisms of imiquimod- and IFN-alpha-mediated antitumor reactivity. J. Immunol., 2012-01-09;188(4):1583-91. 2012-01-09 [PMID: 22231699] (Flow Cytometry, Human)
• Bo Zhang, Hong Shan, Dan Li, Zheng-Ran Li, Kang-Shun Zhu, Zai-Bo Jiang et al. Cisplatin sensitizes human hepatocellular carcinoma cells, but not hepatocytes and mesenchymal stem cells, to TRAIL within a therapeutic window partially depending on the upregulation of DR5 Oncology Reports 2011-02-01 [PMID: 21152876]
• Stary G, Klein I, Kohlhofer S, Koszik F, Scherzer T, Mullauer L, Quendler H, Kohrgruber N, Stingl G Plasmacytoid dendritic cells express TRAIL and induce CD4+ T-cell apoptosis in HIV-1 viremic patients. Blood, 2009-08-18;114(18):3854-63. 2009-08-18 [PMID: 19690337] (Flow Cytometry, Human)
• Kim SM, Lim JY, Park SI, Jeong CH, Oh JH, Jeong M, Oh W, Park SH, Sung YC, Jeun SS Gene therapy using TRAIL-secreting human umbilical cord blood-derived mesenchymal stem cells against intracranial glioma. Cancer Res., 2008-12-01;68(23):9614-23. 2008-12-01 [PMID: 19047138] (Flow Cytometry, Human)
• Secchiero P, Melloni E, Corallini F, Beltrami AP, Alviano F, Milani D, D'Aurizio F, di Iasio MG, Cesselli D, Bagnara GP, Zauli G Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells. Stem Cells, 2008-09-04;26(11):2955-63. 2008-09-04 [PMID: 18772312] (Flow Cytometry, Human)
• Dunn C, Brunetto M, Reynolds G, Christophides T, Kennedy PT, Lampertico P, Das A, Lopes AR, Borrow P, Williams K, Humphreys E, Afford S, Adams DH, Bertoletti A, Maini MK Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage. J. Exp. Med., 2007-03-12;204(3):667-80. 2007-03-12 [PMID: 17353365] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TNFRSF10D
• Uniprot:
  • Q9UBN6()