TR2/NR2C1 Antibody

Western Blot: TR2/NR2C1 Antibody [NBP1-71803] - Whole cell lysate from HeLa (H; 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (5, 15, and 50 mcg for WB) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-TR2 antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. TR2 was also immunoprecipitated by rabbit anti-TR2 antibody NBP1-71804, which recognizes a downstream epitope.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

TR2/NR2C1 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 1 and 50 of human Testicular Receptor 2 using the numbering given in entry NP_003288.2 (GeneID 7181).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR2C1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 2-5 mcg/mg
  • Western Blot 1:2000-1:10000
• Application Notes: Use in Immunohistochemistry reported in scientific literature (PMID 28551284).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TR2/NR2C1 Antibody

• NR2C1
• nuclear receptor subfamily 2 group C member 1
• nuclear receptor subfamily 2, group C isoform
• nuclear receptor subfamily 2, group C, member 1
• Orphan nuclear receptor TR2
• Testicular receptor 2
• TR2 nuclear hormone receptor
• TR2
• TR2-11

Background

This gene encodes a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TR2/NR2C1 Antibody (NBP1-71803)(1)

Publication using NBP1-71803

• Olivares AM, Han Y, Soto D et al. The Nuclear Hormone Receptor Gene Nr2c1 (Tr2) is a Critical Regulator of Early Retina Cell Patterning. Dev. Biol. 2017-05-24 [PMID: 28551284] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: NR2C1
• Uniprot:
  • NP_003288.2(Human)