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TR2/NR2C1 Antibody

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Western Blot: TR2/NR2C1 Antibody [NBP1-71803] - Whole cell lysate from HeLa (H; 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (5, 15, and 50 mcg for WB) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-TR2 antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. TR2 was also immunoprecipitated by rabbit anti-TR2 antibody NBP1-71804, which recognizes a downstream epitope.
Independent Antibodies: Western Blot: TR2/NR2C1 Antibody [NBP1-71803] - Whole cell lysate from HeLa (H; 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (5, 15, and 50 mcg for WB) and mouse NIH3T3 (M; 50 mcg) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

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TR2/NR2C1 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 1 and 50 of human Testicular Receptor 2 using the numbering given in entry NP_003288.2 (GeneID 7181).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR2C1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunoprecipitation 2-5 mcg/mg
  • Western Blot 1:2000-1:10000
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID 28551284).
Publications
Read Publication using
NBP1-71803 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TR2/NR2C1 Antibody

  • NR2C1
  • nuclear receptor subfamily 2 group C member 1
  • nuclear receptor subfamily 2, group C isoform
  • nuclear receptor subfamily 2, group C, member 1
  • Orphan nuclear receptor TR2
  • Testicular receptor 2
  • TR2 nuclear hormone receptor
  • TR2
  • TR2-11

Background

This gene encodes a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Oct‑3/4 at approximately 50 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Western blot shows lysates of TF‑1 human erythroleukemic cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRAIL R1/TNFRSF10A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF347) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TRAIL R1/TNFRSF10A at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.
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TR2/NR2C1 Antibody
NBP1-71803
TR2/NR2C1 Antibody
Species: Hu, Mu
Applications: WB, IHC, IP

Publications for TR2/NR2C1 Antibody (NBP1-71803)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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Bioinformatics

Gene Symbol NR2C1
Uniprot