TR beta 1/NR1A2/Thyroid Hormone Receptor beta Antibody

Western Blot: TR beta 1/NR1A2/Thyroid Hormone Receptor beta Antibody [NB100-93535] - Staining of Zebrafish adult Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Ze
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TR beta 1/NR1A2/Thyroid Hormone Receptor beta Antibody Summary

• Immunogen: Peptide with sequence C-DIGSAPIVNAPEGNKVD corresponding to internal region according to Uniprot Zebrafish NP_571415.1.
• Epitope: C-DIGSAPIVNAPEGNKVD
• Specificity: This antibody is not expected to cross-react to Thra.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: THRB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 2 - 5 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in Zebrafish adult heart lysates (calculated MW of 44.3 kDa band according to NP_571415.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for TR beta 1/NR1A2/Thyroid Hormone Receptor beta Antibody

• avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2
• c-erbA-2
• c-erbA-beta
• ERBA2MGC126109
• ERBA-BETA
• GRTH
• MGC126110
• NR1A2
• NR1A2thyroid hormone receptor, beta (avian erythroblastic leukemia viral (v-erb-a)oncogene homolog 2)
• Nuclear receptor subfamily 1 group A member 2
• oncogene ERBA2
• PRTH
• THR1pituitary resistance to thyroid hormone
• THRB
• THRB1
• THRB2
• thyroid hormone receptor beta 1
• thyroid hormone receptor beta
• thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a)oncogene homolog 2, avian)
• TR beta 1
• v-ErbA2

Background

Thyroid Hormone Receptor beta is encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Defects in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TR beta 1/NR1A2/Thyroid Hormone Receptor beta Antibody (NB100-93535)(1)

Publication using NB100-93535

• Liu YW, Chan WK. Thyroid hormones are important for embryonic to larval transitory phase in zebrafish. Differentiation 2002-03-01 [PMID: 11963654]

Bioinformatics

• Gene Symbol: THRB
• Entrez:
  • 7068(Human)
• Uniprot:
  • NP_571415.1(Zebrafish)