TPMT Antibody (2E7)

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Western Blot: TPMT Antibody (AT2E7) [NBP1-97939] The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1,000). Proteins were visualized using a goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

TPMT Antibody (2E7) Summary

Immunogen
Recombinant human TPMT (1-245aa) purified from E. coli
Specificity
Anti-human TPMT mAb, clone 2E7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TPMT protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TPMT
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
Application Notes
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for TPMT Antibody (2E7)

  • EC 2.1.1.67
  • S-adenosyl-L-methionine:thiopurine S-methyltransferase
  • Thiopurine methyltransferase
  • thiopurine S-methyltransferase

Background

TPMT(Thiopurine S-methyltransferase) is a very important enzyme for the metabolizm of thiopurine compounds. TPMT pharmacogenetics has been studied extensively because of its clinical significance. Many patients got benefited from the knowledge of TPMT genotype-phenotype correlation. Also, TPMT activity exhibits autosomal codominant genetic polymorphism, and patients inheriting TPMT-deficiency are at high risk of potentially fatal hematopoietic toxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TPMT
Uniprot