TPMT Antibody (1D4) - Azide and BSA Free

Western Blot: TPMT Antibody (1D4) [H00007172-M02] - Analysis of TPMT expression in transfected 293T cell line by TPMT monoclonal antibody (M02), clone 1D4. Lane 1: TPMT transfected lysate(28.2 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: TPMT Antibody (1D4) [H00007172-M02] - Analysis of monoclonal antibody to TPMT on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: TPMT Antibody (1D4) [H00007172-M02] - Detection limit for recombinant GST tagged TPMT is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TPMT Antibody (1D4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TPMT (AAH05339, 1 a.a. ~ 245 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDGTRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVNGKTAFHQEQGHQLLKKHLDTFLKGKSGLRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIQEFFTEQNLSYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPRTNIGKFDMIWDRGALVAINPGDRKCYADTMFSLLGKKFQYLLCVLSYDPTKHPGPPFYVPHAEIERLFGKICNIRRLEKVDAFEERHKSWGIDCLFEKLYLLTEK
• Specificity: TPMT - thiopurine S-methyltransferase
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPMT
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TPMT Antibody (1D4) - Azide and BSA Free

• EC 2.1.1.67
• S-adenosyl-L-methionine:thiopurine S-methyltransferase
• Thiopurine methyltransferase
• thiopurine S-methyltransferase
• TPMT

Background

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TPMT
• Entrez:
  • 7172(Human)
• OMIM:
  • 187680(Human)
• Uniprot:
  • AAH05339(Human)