TPMT Antibody (1D4)

Images

 
Western Blot: TPMT Antibody (1D4) [H00007172-M02] - Analysis of TPMT expression in transfected 293T cell line by TPMT monoclonal antibody (M02), clone 1D4. Lane 1: TPMT transfected lysate(28.2 KDa). Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: TPMT Antibody (1D4) [H00007172-M02] - Analysis of monoclonal antibody to TPMT on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: TPMT Antibody (1D4) [H00007172-M02] - Detection limit for recombinant GST tagged TPMT is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
1D4
Clonality
Monoclonal
Host
Mouse

Order Details

TPMT Antibody (1D4) Summary

Immunogen
TPMT (AAH05339, 1 a.a. ~ 246 a.a) full length recombinant protein with GST tag.MDGTRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVNGKTAFHQEQGHQLLKKHLDTFLKGKSGLRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIQEFFTEQNLSYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPRTNIGKFDMIWDRGALVAINPGDRKCYADTMFSLLGKKFQYLLCVLSYDPTKHPGPPFYVPHAEIERLFGKICNIRRLEKVDAFEERHKSWGIDCLFEKLYLLTEK
Specificity
TPMT - thiopurine S-methyltransferase
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TPMT
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TPMT Antibody (1D4)

  • EC 2.1.1.67
  • S-adenosyl-L-methionine:thiopurine S-methyltransferase
  • Thiopurine methyltransferase
  • thiopurine S-methyltransferase

Background

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TPMT Antibody (H00007172-M02) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on TPMT

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol TPMT
Entrez
OMIM
Uniprot

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