TPD52L1/D53 Antibody

Western Blot: TPD52L1/D53 Antibody [NBP1-06091] - Analysis of TPD52L1/D53 using this antibody at 0.1 ug/ml in Human Breast cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TPD52L1/D53 Antibody Summary

• Immunogen: Peptide with sequence C-EPLQGTDEDAVASAD corresponding to internal region according to NP_003278.1, NP_001003395.1 , NP_001003396.1, NP_001003397.1.
• Epitope: C-EPLQGTDEDAVASAD
• Specificity: This antibody is expected to recognise isoforms 1, 3 and 4 (NP_003278.1, NP_001003396.1 and NP_001003397.1 resp.).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TPD52L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 26 kDa band observed in human breast cancer lysates (calculated MW of 22.5 kDa band according to NP_003278.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TPD52L1/D53 Antibody

• D53
• MGC8556
• tumor protein D52-like 1hD53TPD52L2
• tumor protein D52-like 2
• tumor protein D53

Background

This gene encodes a member of the tumor protein D52 (TPD52) family. The encoded protein contains a coiled-coil domain and may form homo- or hetero-dimer with TPD52 family members. The protein is reported to be involved in cell proliferation and calcium signaling. It also interacts with the mitogen-activated protein kinase kinase kinase 5 (MAP3K5/ASK1) and positively regulates MAP3K5-induced apoptosis. Multiple alternatively spliced transcript variants have been observed, but the full-length nature of some variants has not yet been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TPD52L1/D53 Antibody (NBP1-06091)(1)

Publication using NBP1-06091

• Boutros R, Bailey AM, Wilson SH, Byrne JA. Alternative splicing as a mechanism for regulating 14-3-3 binding: interactions between hD53 (TPD52L1) and 14-3-3 proteins. J Mol Biol 2003-09-19 [PMID: 12963375]

Bioinformatics

• Gene Symbol: TPD52L1
• Entrez:
  • 7164(Human)
• Uniprot:
  • NP_003278.1(Human)