Topoisomerase I Overexpression Lysate (Native)


Western Blot: Topoisomerase I Overexpression Lysate (Adult Normal) [NBL1-17194] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Topoisomerase I.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

Topoisomerase I Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC215658 and based on accession number NM_003286. The protein contains a C-terminal DDK tag.
Homo sapiens topoisomerase (DNA) I (TOP1), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
90.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for Topoisomerase I

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Topoisomerase I Overexpression Lysate (Native)

  • DNA topoisomerase 1
  • DNA topoisomerase I
  • EC
  • TOPI
  • topoisomerase (DNA) I
  • type I DNA topoisomerase


This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Topoisomerase I Lysate (NBL1-17194) (0)

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Video Protocols

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Contact Information

Product PDFs


Gene Symbol TOP1

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