Topoisomerase I Antibody (SC69-03)

Western Blot: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Western blot analysis of Topoisomerase I on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goa

Immunocytochemistry/Immunofluorescence: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Staining TOP1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Topoisomerase I antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Analysis of HepG2 cells with TOP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunocytochemistry/Immunofluorescence: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Staining TOP1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Staining TOP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Analysis of paraffin-embedded mouse brain tissue using anti-TOP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Topoisomerase I antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Analysis of paraffin-embedded human large intestine tissue with Rabbit anti-Topoisomerase I antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

Topoisomerase I Antibody (SC69-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Topoisomerase I aa 1-49 / 765. (SwissProt: P11387 Human; SwissProt: Q04750 Mouse)
• Localization: Nucleus, nucleoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TOP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:1000
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Topoisomerase I Antibody (SC69-03)

• DNA topoisomerase 1
• DNA topoisomerase I
• EC 5.6.2.1
• EC 5.99.1.2
• TOP1
• TOPI
• topoisomerase (DNA) I
• Topoisomerase I
• type I DNA topoisomerase

Background

Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then religated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Topoisomerase I Antibody (NBP2-67606)(1)

Publication using NBP2-67606

• Millan C, Prause L, Vallmajo-Martin Q et al. Extracellular Vesicles from 3D Engineered Microtissues Harbor Disease-Related Cargo Absent in EVs from 2D Cultures Advanced healthcare materials 2021-04-22 [PMID: 33890421]

Bioinformatics

• Gene Symbol: TOP1