Topoisomerase I Antibody (SC69-03)

Images

 
Western Blot: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Western blot analysis of Topoisomerase I on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at ...read more
Immunocytochemistry/ Immunofluorescence: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Staining TOP1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Analysis of paraffin-embedded mouse brain tissue using anti-TOP1 antibody. Counter stained with hematoxylin.
Flow Cytometry: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Analysis of HepG2 cells with TOP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; ...read more
Western Blot: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Analysis of TOP1 on different lysates using anti-TOP1 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Jurkat Lane 3: MCF-7
Immunocytochemistry/ Immunofluorescence: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Staining TOP1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunocytochemistry/ Immunofluorescence: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Staining TOP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
SC69-03
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Topoisomerase I Antibody (SC69-03) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within N-terminal human TOP1. (SwissProt: P11387 Human ;SwissProt: Q04750 Mouse)
Localization
Nucleus, nucleoplasm.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
TOP1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
Publications
Read Publication using NBP2-67606.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Topoisomerase I Antibody (SC69-03)

  • DNA topoisomerase 1
  • DNA topoisomerase I
  • EC 5.99.1.2
  • TOPI
  • topoisomerase (DNA) I
  • type I DNA topoisomerase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Topoisomerase I Antibody (NBP2-67606)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Topoisomerase I Antibody (NBP2-67606) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for Topoisomerase I Antibody (NBP2-67606)

Discover related pathways, diseases and genes to Topoisomerase I Antibody (NBP2-67606). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol TOP1