TOP2B Antibody (681417) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, K562 human chronic myelogenous leukemia cell line, and CH-1 mouse B cell lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse TOP2B Monoclonal Antibody (Catalog # MAB6348) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TOP2B at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TOP2B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse TOP2B Monoclonal Antibody (Catalog # MAB6348) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: 681417
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TOP2B Antibody (681417) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TOP2B
  Phe1192-Asp1621
  Accession # Q02880
• Specificity: Detects human TOP2B in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 75% cross-reactivity with recombinant mouse TOP2B and no cross-reactivity with recombinant human TOP2A is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TOP2B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TOP2B Antibody (681417) [Unconjugated]

• antigen MLAA-44
• DNA topoisomerase 2-beta
• DNA topoisomerase II beta
• DNA topoisomerase II, 180 kD
• DNA topoisomerase II, beta isozyme
• EC 5.99.1.3
• TOP2B
• top2beta
• TOPIIB
• topo II beta
• topoisomerase (DNA) II beta (180kD)
• topoisomerase (DNA) II beta 180kDa
• topoisomerase II beta
• topoisomerase IIb
• U937 associated antigen

Background

TOP2B (DNA Topoisomerase II beta) is a 180-185 kDa member of the type IIA subfamily, topoisomerase family of molecules. It is ubiquitously expressed, and represents the larger of two known topoisomerases (the 2A/a-form being 170 kDa in size). TOP2A is essential for cell division, while TOP2B is active postmitotically. In an ATP-dependent manner, homodimeric TOP2B relives torsional stress created in DNA during transcription or as a consequence of replication. TOP2B first induces cleavage of double-stranded DNA, creating a space that allows for the physical repositioning of chromatin and a reduction in tension. This is followed by closure and ligation of the cleaved ends to recreate the original DNA structure. Human TOP2B is 1626 amino acids (aa) in length. It contains an ATPase domain (aa 101-201), an NES (aa 1034-1044), more that 30 Ser/Thr phosphorylation sites and a C-terminal DTHCT region (aa 1508-1611). There is one splice variant that shows a deletion of aa 24-28. Over aa 1187-1621, human TOP2B shares 91% aa identity with mouse TOP2B.               

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TOP2B Antibody (MAB6348)(3)

Publications using MAB6348

• T Magdy, Z Jiang, M Jouni, H Fonoudi, D Lyra-Leite, G Jung, M Romero-Tej, HH Kuo, KA Fetterman, M Gharib, BT Burmeister, M Zhao, Y Sapkota, CJ Ross, BC Carleton, D Bernstein, PW Burridge RARG variant predictive of doxorubicin-induced cardiotoxicity identifies a cardioprotective therapy Cell Stem Cell, 2021-09-15;0(0):. 2021-09-15 [PMID: 34525346] (Western Blot, Human)
• Julien Oury, Yun Liu, Ana Töpf, Slobodanka Todorovic, Esthelle Hoedt, Veeramani Preethish-Kumar et al. MACF1 links Rapsyn to microtubule- and actin-binding proteins to maintain neuromuscular synapses Journal of Cell Biology 2019-05-06 [PMID: 30842214]
• Ka C. Lee, Rebecca L. Bramley, Ian G. Cowell, Graham H. Jackson, Caroline A. Austin Proteasomal inhibition potentiates drugs targeting DNA topoisomerase II Biochemical Pharmacology 2016-03-01 [PMID: 26794000]

Bioinformatics

• Gene Symbol: TOP2B
• Uniprot:
  • Q02880(Human)