TOP1MT Antibody

Immunocytochemistry/Immunofluorescence: TOP1MT Antibody [NBP1-89473] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.

Immunohistochemistry-Paraffin: TOP1MT Antibody [NBP1-89473] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TOP1MT Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GYCILDGHQEKIGNFKIEPPGLFRGRGDHPKMGMLKRRITPEDVVINCSRDSKIPEPPAGHQWKEVRSDNTVTWLAAWTESVQNSIKYIMLNPCSKLKGETAWQKFETARRLRGFVDEIRSQYRADWKSREMKTRQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TOP1MT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TOP1MT Protein (NBP1-89473PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (83%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TOP1MT Antibody

• mitochondrial DNA topoisomerase I
• mitochondrial
• topoisomerase (DNA) I, mitochondrial

Background

The TOP1MT gene encodes a mitochondrial DNA topoisomerase, an enzyme that controls and alters the topologic states of DNAduring transcription. This enzyme catalyzes the transient breaking and rejoining of DNA which allows the strands topass through one another

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TOP1MT Antibody (NBP1-89473)(1)

Publication using NBP1-89473

• Nath S, Caron NS, May L et al. Functional characterization of variants of unknown significance in a spinocerebellar ataxia patient using an unsupervised machine learning pipeline Human genome variation 2022-04-14 [PMID: 35422034] (ICC/IF)

Bioinformatics

• Gene Symbol: TOP1MT