TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted

Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472] - Formalin-fixed, paraffin-embedded human Gallbladder stained with TOP1MT Mouse Monoclonal Antibody (TOP1MT/488).

Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472] - Human Pancreas stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).

Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted [NBP2-48472] - Human Melanoma stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).

Western Blot Analysis of Jurkat cell lysate using TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted.

Flow Cytometric Analysis of PFA-fixed MCF-7 cells. TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Immunofluorescent analysis of PFA-fixed MCF-7 cells. TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted followed by goat anti-mouse IgG-CF488 (green); nuclear counterstain (RedDot).

• Reactivity: Hu
• Applications: IHC
• Clone: TOP1MT/488
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Recombinant full-length human TOP1MT protein (Uniprot: Q969P6 )
• Localization: Cytoplasmic (Mitochondria)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TOP1MT
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for TOP1MT Antibody (TOP1MT/488) - IHC-Prediluted

• mitochondrial DNA topoisomerase I
• mitochondrial
• topoisomerase (DNA) I, mitochondrial

Background

The TOP1MT gene encodes a mitochondrial DNA topoisomerase, an enzyme that controls and alters the topologic states of DNAduring transcription. This enzyme catalyzes the transient breaking and rejoining of DNA which allows the strands topass through one another

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TOP1MT