| Reactivity | HuSpecies Glossary |
| Applications | Flow, IHC, IHC-P |
| Clone | TOP1MT/488 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 488 |
| Immunogen | Recombinant full-length human TOP1MT protein |
| Localization | Cytoplasmic (Mitochondria) |
| Specificity | DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TOP1MT |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-47793AF350 | |
| Alexa Fluor 405 | NBP2-47793AF405 | |
| Alexa Fluor 488 | NBP2-47793AF488 | |
| Alexa Fluor 532 | NBP2-47793AF532 | |
| Alexa Fluor 594 | NBP2-47793AF594 | |
| Alexa Fluor 647 | NBP2-47793AF647 | |
| Alexa Fluor 700 | NBP2-47793AF700 | |
| Alexa Fluor 750 | NBP2-47793AF750 | |
| Allophycocyanin | NBP2-47793APC | |
| Biotin | NBP2-47793B | |
| DyLight 350 | NBP2-47793UV | |
| DyLight 405 | NBP2-47793V | |
| DyLight 488 | NBP2-47793G | |
| DyLight 550 | NBP2-47793R | |
| DyLight 594 | NBP2-47793DL594 | |
| DyLight 650 | NBP2-47793C | |
| DyLight 680 | NBP2-47793FR | |
| DyLight 755 | NBP2-47793IR | |
| FITC | NBP2-47793F | |
| HRP | NBP2-47793H | |
| Janelia Fluor 549 | NBP2-47793JF549 | |
| Janelia Fluor 646 | NBP2-47793JF646 | |
| PE | NBP2-47793PE | |
| PerCP | NBP2-47793PCP |
Diseases for TOP1MT Antibody (NBP2-47793G)Discover more about diseases related to TOP1MT Antibody (NBP2-47793G).
| Pathways for TOP1MT Antibody (NBP2-47793G)View related products by pathway.
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PTMs for TOP1MT Antibody (NBP2-47793G)Learn more about PTMs related to TOP1MT Antibody (NBP2-47793G).
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| Gene Symbol | TOP1MT |
![Western Blot: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Analysis of TOP1 on different lysates using anti-TOP1 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Jurkat Lane 3: MCF-7](http://images.novusbio.com/images2/Topoisomerase I Antibody (SC69-03)-Western Blot-NBP2-67606-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Staining TOP1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Topoisomerase-I-Antibody-(SC69-03)-Immunocytochemistry-Immunofluorescence-NBP2-67606-img0004.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody (SC69-03) [NBP2-67606] - Analysis of paraffin-embedded mouse brain tissue using anti-TOP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Topoisomerase-I-Antibody-(SC69-03)-Immunohistochemistry-Paraffin-NBP2-67606-img0005.jpg)
![Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody NB600-335 at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/images2/c-Myc-Antibody-Western-Blot-NB600-335-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 20 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the Alexa Fluor 488 format of this antibody.](http://images.novusbio.com/images2/c-Myc-Antibody-Immunocytochemistry-Immunofluorescence-NB600-335-img0007.jpg)
![Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.4 ug/mL (1:25,000).](http://images.novusbio.com/images2/c-Myc-Antibody-Western-Blot-NB600-335-img0005.jpg)
![Western Blot: LARP6 Antibody [H00055323-B01P] - Analysis of LARP6 expression in transfected 293T cell line by LARP6 polyclonal antibody. Lane 1: LARP6 transfected lysate(54.01 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/LARP6 Antibody-Western Blot-H00055323-B01P-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LARP6 Antibody [H00055323-B01P] - Analysis of purified antibody to LARP6 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/images2/LARP6-Antibody-Immunocytochemistry-Immunofluorescence-H00055323-B01P-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ATP8A2 Antibody [NBP2-55197] - Staining of human cell line PC-3 shows localization to plasma membrane.](http://images.novusbio.com/images2/ATP8A2 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55197-img0001.jpg)
![Simple Western: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Simple Western lane view shows a specific band for mtTFA in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/mtTFA-Antibody-(18G102B2E11)-Simple-Western-NBP1-71648-img0008.jpg)
![Knockdown Validated: mtTFA Antibody (18G102B2E11) [NBP1-71648] - MtDNA depletion and reversible impaired immune functions in THP-1A cells. A and B, Treatment with 50A ng/mL ethidium bromide (EtBr) for 8A weeks. Transfection with 30A nmol/L negative or TFAM siRNA for 8A days. TFAM protein relative toI2-actin. D, MtDNA levels. E, LPS-induced TNF-I+-and IL-8 release. Image collected and cropped by Cite Ab from the following publication (Mitochondrial DNA depletion induces innate immune dysfunction rescued by IFN-g. J Allergy Clin Immunol (2017)) licensed under a CC-BY licence.](http://images.novusbio.com/images2/mtTFA-Antibody-(18G102B2E11)-Western-Blot-NBP1-71648-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Immunostaining in HeLa cells. C1: DAPI staining 2 nuclei, C2: anti-mtTFA stain with Alexa Fluor 488 secondary ab (green), C3: Mitotracker Red staining mitochondria. Image courtesy of Elizabeth Wang.](http://images.novusbio.com/images2/mtTFA-Antibody-(18G102B2E11)-Immunocytochemistry-Immunofluorescence-NBP1-71648-img0003.jpg)
![Immunohistochemistry-Paraffin: ROS Antibody [NBP2-13245] - Staining ROS1 in human ovarian cancer tissue.Image submitted by a verified customer review.](http://images.novusbio.com/images2/ROS-Antibody-Immunohistochemistry-Paraffin-NBP2-13245-img0002.jpg)
![Immunohistochemistry-Paraffin: ROS Antibody [NBP2-13245] Staining of human kidney shows moderate membranous positivity in cells in glomeruli.](http://images.novusbio.com/images/ROS Antibody-Immunohistochemistry-Paraffin-NBP2-13245-img0001.jpg)
![Western Blot: DNA Polymerase gamma Antibody [NBP1-33633] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase gamma antibody diluted by 1:1000.](http://images.novusbio.com/images2/DNA-Polymerase-gamma-Antibody-Western-Blot-NBP1-33633-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: DNA Polymerase gamma Antibody [NBP1-33633] - analysis of methanol-fixed HeLa.](http://images.novusbio.com/images2/DNA-Polymerase-gamma-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33633-img0008.jpg)
![Immunohistochemistry-Paraffin: DNA Polymerase gamma Antibody [NBP1-33633] - Paraffin-embedded Skeletal Muscle, using antibody 10 ug/ml.](http://images.novusbio.com/images2/DNA Polymerase gamma Antibody-Immunohistochemistry-Paraffin-NBP1-33633-img0005.jpg)
![Western Blot: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody (M01), clone 4D10.Lane 1: MUTYH transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/MYH-Antibody-(4D10)-Western-Blot-H00004595-M01-img0009.jpg)
![Western Blot: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi ( Cat # H00004595-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody (M01), clone 4D10 (Cat # H00004595-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/images2/MYH-Antibody-(4D10)-Western-Blot-H00004595-M01-img0006.jpg)
![Western Blot: MYH Antibody (4D10) [H00004595-M01] - MUTYH monoclonal antibody (M01), clone 4D10 Analysis of MUTYH expression in Hela S3 NE.](http://images.novusbio.com/images2/MYH-Antibody-(4D10)-Western-Blot-H00004595-M01-img0008.jpg)
![Western Blot: E2F6 Antibody (1F7-A9) [H00001876-M02] - Western Blot analysis of E2F6 expression in A-549 ( Cat # L025V1 ).](http://images.novusbio.com/images2/E2F6-Antibody-(1F7-A9)-Western-Blot-H00001876-M02-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: E2F6 Antibody (1F7-A9) [H00001876-M02] - Analysis of monoclonal antibody to E2F6 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/E2F6-Antibody-(1F7-A9)-Immunocytochemistry-Immunofluorescence-H00001876-M02-img0007.jpg)
![Western Blot: E2F6 Antibody (1F7-A9) [H00001876-M02] - Western Blot analysis of E2F6 expression in PC-12(Cat # L012V1 ).](http://images.novusbio.com/images2/E2F6-Antibody-(1F7-A9)-Western-Blot-H00001876-M02-img0001.jpg)
![Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of E2F1 on HepG2 cells lysates using anti-E2F1 antibody at 1/1,000 dilution.](http://images.novusbio.com/images2/E2F-1 Antibody (JJ092-02)-Western Blot-NBP2-67899-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/E2F-1-Antibody-(JJ092-02)-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0004.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded mouse pancreas tissue using anti-E2F1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/E2F-1-Antibody-(JJ092-02)-Immunohistochemistry-Paraffin-NBP2-67899-img0006.jpg)
![Western Blot: APEX2 Antibody [NB100-56625] - Western blot analysis of APEXL2 in HeLa cell lysates using NB100-56625 at a dilution of 1:500.](http://images.novusbio.com/images2/APEX2 Antibody-Western Blot-NB100-56625-img0001.jpg)