![Western Blot: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Analysis of human Jurkat cell lysate using TOP1MT Antibody (TOP1MT/488).](http://images.novusbio.com/fullsize2/TOP1MT-Antibody-(TOP1MT-488)---Azide-and-BSA-Free-Western-Blot-NBP2-47793-img0005.jpg "Western Blot: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Analysis of human Jurkat cell lysate using TOP1MT Antibody (TOP1MT/488).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, CyTOF-ready, ICC/IF |
| Clone | TOP1MT/488 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Recombinant full-length human TOP1MT protein |
| Localization | Cytoplasmic (Mitochondria) |
| Specificity | DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TOP1MT |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
![Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/images2/c-Myc-Antibody-(9E10)-Flow-Cytometry-NB600-302-img0014.jpg)
![Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/images2/c-Myc-Antibody-(9E10)-Flow-Cytometry-NB600-302-img0019.jpg)
![Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY licence.](http://images.novusbio.com/images2/c-Myc-Antibody-(9E10)-Western-Blot-NB600-302-img0022.jpg)
![Immunohistochemistry-Paraffin: ATP8A2 Antibody [NBP1-88899] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/images2/ATP8A2-Antibody-Immunohistochemistry-Paraffin-NBP1-88899-img0005.jpg)
![Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Alcohol feeding significantly decreased TFAM expression in rat liver. This image was submitted by a customer review.](http://images.novusbio.com/images2/mtTFA-Antibody-(18G102B2E11)-Western-Blot-NBP1-71648-img0009.jpg)
![Knockdown Validated: mtTFA Antibody (18G102B2E11) [NBP1-71648] - MtDNA depletion and reversible impaired immune functions in THP-1A cells. A and B, Treatment with 50A ng/mL ethidium bromide (EtBr) for 8A weeks. Transfection with 30A nmol/L negative or TFAM siRNA for 8A days. TFAM protein relative toI2-actin. D, MtDNA levels. E, LPS-induced TNF-I+-and IL-8 release. Image collected and cropped by Citeab from the following publication (Mitochondrial DNA depletion induces innate immune dysfunction rescued by IFN-g. J Allergy Clin Immunol (2017)) licensed under a CC-BY licence.](http://images.novusbio.com/images2/mtTFA-Antibody-(18G102B2E11)-Western-Blot-NBP1-71648-img0010.jpg)
![Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Induction of mitochondrial biogenesis following exposure of THP-1 cells to LPS. THP-1 cells were incubated with LPS (100 ng/ml) for 0AE](http://images.novusbio.com/images2/mtTFA-Antibody-(18G102B2E11)-Western-Blot-NBP1-71648-img0013.jpg)
![Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626] - Analysis of Carcinoma Human lung tissue.](http://images.novusbio.com/images2/ROS-Antibody-(OTI1F3)-Immunohistochemistry-NBP2-45626-img0001.jpg)
![Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626] - Analysis of Carcinoma Human lung tissue.](http://images.novusbio.com/images2/ROS-Antibody-(OTI1F3)-Immunohistochemistry-NBP2-45626-img0002.jpg)
![Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626] - Analysis of Human carcinoma lung tissue.](http://images.novusbio.com/images2/ROS-Antibody-(OTI1F3)-Immunohistochemistry-NBP2-45626-img0003.jpg)
![Sandwich ELISA: E2F6 Antibody (1F7-A9) [H00001876-M02] - Detection limit for recombinant GST tagged E2F6 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/images2/E2F6-Antibody-(1F7-A9)-Sandwich-ELISA-H00001876-M02-img0008.jpg)
![Western Blot: E2F6 Antibody (1F7-A9) [H00001876-M02] - Western Blot analysis of E2F6 expression in PC-12(Cat # L012V1 ).](http://images.novusbio.com/images2/E2F6-Antibody-(1F7-A9)-Western-Blot-H00001876-M02-img0001.jpg)
![Western Blot: E2F6 Antibody (1F7-A9) [H00001876-M02] - Western Blot analysis of E2F6 expression in HepG2 ( Cat # L019V1 ).](http://images.novusbio.com/images2/E2F6-Antibody-(1F7-A9)-Western-Blot-H00001876-M02-img0002.jpg)
![Flow Cytometry: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of Hela cells with E2F1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/images2/E2F-1-Antibody-(JJ092-02)-Flow-Cytometry-NBP2-67899-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/E2F-1-Antibody-(JJ092-02)-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/E2F-1-Antibody-(JJ092-02)-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0003.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for TOP1MT Antibody (NBP2-47793)Discover more about diseases related to TOP1MT Antibody (NBP2-47793).
| Pathways for TOP1MT Antibody (NBP2-47793)View related products by pathway.
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PTMs for TOP1MT Antibody (NBP2-47793)Learn more about PTMs related to TOP1MT Antibody (NBP2-47793).
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| Gene Symbol | TOP1MT |
![Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Formalin-fixed, paraffin-embedded human Pancreas stained with TOP1MT Mouse Monoclonal Antibody (TOP1MT/488).](http://images.novusbio.com/fullsize2/TOP1MT-Antibody-(TOP1MT-488)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47793-img0011.jpg "Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Formalin-fixed, paraffin-embedded human Pancreas stained with TOP1MT Mouse Monoclonal Antibody (TOP1MT/488).")
![Flow Cytometry: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - An intracellular stain was performed on MCF7 cells with TOP1MT Antibody [TOP1MT/488] NBP2-47793AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize2/TOP1MT-Antibody-(TOP1MT-488)---Azide-and-BSA-Free-Flow-Cytometry-NBP2-47793-img0010.jpg "Flow Cytometry: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - An intracellular stain was performed on MCF7 cells with TOP1MT Antibody [TOP1MT/488] NBP2-47793AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Human Pancreas stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).](http://images.novusbio.com/fullsize2/TOP1MT-Antibody-(TOP1MT-488)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47793-img0006.jpg "Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Human Pancreas stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).")
![Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Human Melanoma stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).](http://images.novusbio.com/fullsize2/TOP1MT-Antibody-(TOP1MT-488)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47793-img0007.jpg "Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Human Melanoma stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).")
![Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Human Gallbladder stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).](http://images.novusbio.com/fullsize2/TOP1MT-Antibody-(TOP1MT-488)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47793-img0008.jpg "Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Human Gallbladder stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).")
![Flow Cytometry: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Analysis of PFA-fixed MCF-7 cells using TOP1MT Antibody (TOP1MT/488).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize2/TOP1MT-Antibody-(TOP1MT-488)---Azide-and-BSA-Free-Flow-Cytometry-NBP2-47793-img0003.jpg "Flow Cytometry: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Analysis of PFA-fixed MCF-7 cells using TOP1MT Antibody (TOP1MT/488).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Flow (Intracellular): TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - An intracellular stain was performed on U2OS cells with TOP1MT Antibody [TOP1MT/488] NBP2-47793AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize2/TOP1MT-Antibody-(TOP1MT-488)---Azide-and-BSA-Free-Flow-(Intracellular)-NBP2-47793-img0004.jpg "Flow (Intracellular): TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - An intracellular stain was performed on U2OS cells with TOP1MT Antibody [TOP1MT/488] NBP2-47793AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Immunocytochemistry/Immunofluorescence: Topoisomerase I Antibody [NBP1-90365] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli fibrillar center. Antibody staining is shown in green.](http://images.novusbio.com/images2/Topoisomerase-I-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90365-img0014.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Analysis in human placenta and skeletal muscle tissues. Corresponding TOP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0015.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/images2/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0016.jpg)
![Western Blot: LARP6 Antibody [H00055323-B01P] - Analysis of LARP6 expression in transfected 293T cell line by LARP6 polyclonal antibody. Lane 1: LARP6 transfected lysate(54.01 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/LARP6-Antibody-Western-Blot-H00055323-B01P-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LARP6 Antibody [H00055323-B01P] - Analysis of purified antibody to LARP6 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/images2/LARP6-Antibody-Immunocytochemistry-Immunofluorescence-H00055323-B01P-img0003.jpg)
![Immunohistochemistry-Paraffin: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with human skeletal muscle at concentration 10 ug/ml.](http://images.novusbio.com/images2/DNA-Polymerase-gamma-Antibody-Immunohistochemistry-Paraffin-NBP1-52300-img0003.jpg)
![Western Blot: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with (30 ug of whole cell lysate). A: 293T. 5% SDS PAGE. POLG antibody diluted at 1:5000.](http://images.novusbio.com/images2/DNA-Polymerase-gamma-Antibody-Western-Blot-NBP1-52300-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with methanol-fixed HeLa cell at 1:200 dilution.](http://images.novusbio.com/images2/DNA-Polymerase-gamma-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-52300-img0002.jpg)
![Western Blot: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody (M01), clone 4D10.Lane 1: MUTYH transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/MYH-Antibody-(4D10)-Western-Blot-H00004595-M01-img0009.jpg)
![Knockdown Validated: MYH Antibody (4D10) [H00004595-M01] - Simultaneous suppression of MTH1 and MYH was efficiently achieved using a two-vector system. To investigate the knockdown efficiency at protein level, western blot analysis was performed after 96 hrs of Dox treatment of cells with shRNA set1 (d). Data were double normalized with GAPDH and NT-shRNA control samples and expressed as percentage. The graphs represent mean +/-s.e.m. from three independent experiments. P-values were calculated using one-way analysis of variance (ANOVA). *P<0.05, **P<0.01, ***P<0.001, NS, not significant. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/oncsis201672) licensed under a CC-BY licence.](http://images.novusbio.com/images2/MYH-Antibody-(4D10)-Knockdown-Validated-H00004595-M01-img0011.jpg)
![Immunoprecipitation: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/images2/MYH-Antibody-(4D10)-Immunoprecipitation-H00004595-M01-img0005.jpg)
![Western Blot: APEX2 Antibody [NB100-56625] - Western blot analysis of APEXL2 in HeLa cell lysates using NB100-56625 at a dilution of 1:500.](http://images.novusbio.com/images2/APEX2 Antibody-Western Blot-NB100-56625-img0001.jpg)
