TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free

Images

 
Western Blot: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Analysis of human Jurkat cell lysate using TOP1MT Antibody (TOP1MT/488).
Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Formalin-fixed, paraffin-embedded human Pancreas stained with TOP1MT Mouse Monoclonal Antibody (TOP1MT/488).
Flow Cytometry: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - An intracellular stain was performed on MCF7 cells with TOP1MT Antibody [TOP1MT/488] NBP2-47793AF488 (blue) and a matched isotype control ...read more
Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Human Pancreas stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Human Melanoma stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Human Gallbladder stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
Flow Cytometry: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Analysis of PFA-fixed MCF-7 cells using TOP1MT Antibody (TOP1MT/488).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Flow (Intracellular): TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - An intracellular stain was performed on U2OS cells with TOP1MT Antibody [TOP1MT/488] NBP2-47793AF488 (blue) and a matched isotype ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, CyTOF-ready, ICC/IF
Clone
TOP1MT/488
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free Summary

Immunogen
Recombinant full-length human TOP1MT protein
Localization
Cytoplasmic (Mitochondria)
Specificity
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TOP1MT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunofluorescence : 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml
  • Western Blot
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Notes

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A or G prepared in 10 mM PBS WITHOUT BSA & azide.

Alternate Names for TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free

  • mitochondrial DNA topoisomerase I
  • mitochondrial
  • topoisomerase (DNA) I, mitochondrial

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TOP1MT