TOMM22 Antibody (1C9-2)

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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ICC/IF
Clone
1C9-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TOMM22 Antibody (1C9-2) Summary

Immunogen
Membrane fraction from Vero (monkey kidney-derived) cells.
Localization
Mitochondrial
Specificity
TOMM22 (1C9-2)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TOMM22
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
ICC: Use at an assay dependent dilution. Western Blot: Use at a concentration of 0.5 - 1.0 ug/ml. Detects a band of approximately 22 kDa. Optimal dilutions/concentrations should be determined by the end user.
Control
Raji Whole Cell Lysate
TOMM22 Overexpression Lysate

Reactivity Notes

Cross-reacts with Human and Monkey.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4) and 1.0% BSA
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for TOMM22 Antibody (1C9-2)

  • 1C9-2
  • hTom22
  • mitochondrial import receptor subunit TOM22 homolog
  • mitochondrial import receptor Tom22
  • MST065
  • TOM22MSTP065
  • Translocase of outer membrane 22 kDa subunit homolog
  • translocase of outer mitochondrial membrane 22 homolog (yeast)

Background

Tom22 has a negatively charged N-terminal region exposed to the cytosol, a putative transmembrane region, and a C-terminal intermembrane space region with little negative charge. The following functions can be assigned to the three domains of Tom22: the cytosolic N-terminal domain plays a dual role, specifically important for presequence binding; the intermembrane space domain provides a trans binding site for presequences, and the single membrane anchor of Tom22 is crucial for the integrity of the GIP (general import gene) complex. In the absence of this membrane anchor, the GIP complex dissociates into small core complexes containing a dimer of Tom40 and the three small Toms. A preprotein is stably arrested and accumulated in the GIP complex by Tom40 and Tom22.4 Such a 100 kDa core complex probably contains a single channel that retains the basic channel properties but is already open in the absence of preproteins. In contrast, in the presence of Tom22, the wild-type GIP complex contains tightly regulated channels (probably three channels). Tom22 apparently represents a component of the machinery that controls the gate. The cytosolic domains of Tom22 and Tom20 are believed to form the major part of a cis site, which mediates the import of all preproteins known to use the general import machinery of mitochondria. The preprotein is then routed through the Tom complex translocation channel and transferred to a trans site on the intermembrane space (IMS) side of the outer membrane. The inter-membrane space exposed segment of Tom40 and the C-terminal tail of Tom22 may contribute to the trans-site. Matrix-targeted proteins are further transferred to the matrix through Product Information import machinery in the inner membrane. The TOM complex of mammalian mitochondria resembles the fungal Tom complex, but is distinct from the plant TOM system. Thus, while unique components of the mammalian mitochondrial import system have been identified (e.g. TOM34 and metaxin), Tom22, and Tom37 have not been identified in plant mitochondria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TOMM22 Antibody (NB120-10436) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol TOMM22
Entrez