![Western Blot: TOMM20 Antibody (1B2) [NBP2-60248] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tomm20 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysate Lane 3.: A431 cell lysate Lane 4.: K562 cell lysate Lane 5.: A549 cell lysate Lane 6.: 293T cell lysate Lane 7.: MCF7 cell lysate Lane 8.: SK-OV-3 cell lysate Lane 9.: PC3 cell lysate](http://images.novusbio.com/fullsize/TOMM20-Antibody-1B2-Western-Blot-NBP2-60248-img0001.jpg "Western Blot: TOMM20 Antibody (1B2) [NBP2-60248] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tomm20 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysate Lane 3.: A431 cell lysate Lane 4.: K562 cell lysate Lane 5.: A549 cell lysate Lane 6.: 293T cell lysate Lane 7.: MCF7 cell lysate Lane 8.: SK-OV-3 cell lysate Lane 9.: PC3 cell lysate")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clone | 1B2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Mouse TOMM20 Antibody (1B2) - BSA Free (NBP2-60248) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant human Tomm20 (25-145aa) purified from E. coli |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TOMM20 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 10% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
WB | Mouse | 05/29/2023 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | TOMM20 |
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (1B2) [NBP2-60248] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/TOMM20-Antibody-1B2-Immunocytochemistry-Immunofluorescence-NBP2-60248-img0002.jpg "Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (1B2) [NBP2-60248] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).")
![Flow Cytometry: TOMM20 Antibody (1B2) [NBP2-60248] - Analysis of Tomm20 in HeLa cells. The cell was stained, at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray)](http://images.novusbio.com/fullsize/TOMM20-Antibody-1B2-Flow-Cytometry-NBP2-60248-img0005.jpg "Flow Cytometry: TOMM20 Antibody (1B2) [NBP2-60248] - Analysis of Tomm20 in HeLa cells. The cell was stained, at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray)")
![Western Blot: TOMM20 Antibody (1B2) [NBP2-60248] - Analysis of mouse bone marrow derived macrophages using TOMM20 at 1:1000 dilution. A single band observed around the 15kDa ladder. The image is captured using chemiluminescence imaging system. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-60248_mouse-tomm20-mab-1b2-bsa-free-western-blot-562023153736.jpg "Western Blot: TOMM20 Antibody (1B2) [NBP2-60248] - Analysis of mouse bone marrow derived macrophages using TOMM20 at 1:1000 dilution. A single band observed around the 15kDa ladder. The image is captured using chemiluminescence imaging system. Image from verified customer review.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")
