TOE1 Antibody (1C12)

Western Blot: TOE1 Antibody (1C12) [H00114034-M02] - Analysis of TOE1 expression in transfected 293T cell line by TOE1 monoclonal antibody (M02), clone 1C12. Lane 1: TOE1 transfected lysate(56.548 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: TOE1 Antibody (1C12) [H00114034-M02] - Analysis of monoclonal antibody to TOE1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: TOE1 Antibody (1C12) [H00114034-M02] - Analysis of monoclonal antibody to TOE1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1.5 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TOE1 Antibody (1C12) Summary

• Immunogen: TOE1 (AAH09364, 1 a.a. ~ 510 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAADSDDGAVSAPAASDGGVSKSTTSGEELVVQVPVVDVQSNNFKEMWPSLLLAIKTANFVAVDTELSGLGDRKSLLNQCIEERYKAVCHAARTRSILSLGLACFKRQPDKGEHSYLAQVFNLTLLCMEEYVIEPKSVQFLIQHGFNFNQQYAQGIPYHKGNDKGDESQSQSVRTLFLELIRARRPLVLHNGLIDLVFLYQNFYAHLPESLGTFTADLCEMFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLTLEFCNYPSSMRDHIDYRCCLPPATHRPHPTSICDNFSAYGWCPLGPQCPQSHDIDLIIDTDEAAAEDKRRRRRRREKRKRALLNLPGTQTSGEAKDGPPKKQVCGDSIKPEETEQEVAADETRNLPHSKQGNKNDLEMGIKAARPEIADRATSEVPGSQASPNPVPGDGLHRAGFDAFMTGYVMAYVEVSQGPQPCSSGPWLPECHNKVYLSGKAVPLTVAKSQFSRSSKAHNQKMKLTWGSS
• Specificity: TOE1 (1C12)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TOE1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin) and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TOE1 Antibody (1C12)

• FLJ13949
• target of EGR1 protein 1
• target of EGR1, member 1 (nuclear)

Background

TOE1 inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediatesthe inhibitory growth effect of EGR1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TOE1
• Entrez:
  • 114034(Human)
• Uniprot:
  • AAH09364(Human)