Kua-UEV Antibody

Product Discontinued

Kua-UEV Antibody Summary

• Immunogen: Peptide with sequence C-AYKFRTHSPEALE corresponding to internal region according to NP_954580.1, NP_001155977.1, NP_954673.1.
• Specificity: This antibody is expected to recognise both reported isoforms of KUA: isoform 1 (NP_954580.1) and isoform 2 (NP_001155977.1). This antibody is also expected to recognize the readthrough transcript product KUA-UEV (NP_954673.1, GeneID 387522).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TMEM189-UBE2V1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments in human HEK293, HepG2, K562 lysates gave no specific signal but low background.
• Control:

  K-562 Whole Cell Lysate
  Uev1a/UBE2V1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Kua-UEV Antibody

• CROC1
• CROC-1
• CROC1B
• CROC-1B
• Kua-UEV
• TMEM189-UBE2V1 fusion protein
• TMEM189-UBE2V1 readthrough transcript
• TMEM189-UBE2V1 readthrough
• TRAF6-regulated IKK activator 1 beta Uev1A
• transmembrane protein 189-ubiquitin-conjugating enzyme E2 variant 1read-through
• UBE2V
• UEV1
• UEV-1

Background

Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene is located in the nucleus and can cause transcriptional activation of the human FOS proto-oncogene. It is thought to be involved in the control of differentiation by altering cell cycle behavior. Multiple alternatively spliced transcripts encoding different isoforms have been described for this gene. A pseudogene has been identified which is also located on chromosome 20. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (Kua-UEV), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kua-UEV Antibody (NB100-1024)(1)

Publication using NB100-1024

• Thomson TM, Lozano JJ, Loukili N et al. Fusion of the human gene for the polyubiquitination coeffector UEV1 with Kua, a newly identified gene. Genome Res 2000-11-01 [PMID: 11076860]

Bioinformatics

• Gene Symbol: TMEM189-UBE2V1
• Entrez:
  • 387521(Human)
  • 387522(Human)