TMEFF1/Tomoregulin-1 Antibody

TMEFF1/Tomoregulin-1 was detected in perfusion fixed frozen sections of mouse spinal cord using 15 µg/mL Goat Anti-Mouse TMEFF1/Tomoregulin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1995) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TMEFF1/Tomoregulin-1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TMEFF1/Tomoregulin‑1
  Ser37-Thr320
  Accession # Q6PFE7
• Specificity: Detects mouse TMEFF1/Tomoregulin‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human (rh) TMEFF1 is observed and less than 5% cross-reactivity with rhTMEFF2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TMEFF1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TMEFF1/Tomoregulin-1 Antibody

• C9orf2
• H7365member 1
• TMEFF1
• Tomoregulin1
• Tomoregulin-1
• TR-1
• Transmembrane protein with EGF-like and one follistatin-like domain
• transmembrane protein with EGF-like and two follistatin-like domains 1

Background

TMEFF1, also known as tomoregulin 1 is a type I transmembrane glycoprotein that contains two follistatin modules and an EGF domain in its extracellular domain, a transmembrane domain and a short cytoplasmic tail. The extracellular domain can be released as a soluble protein. TMEFF1 selectively regulates nodal but not activin signaling through direct binding to the nodal co-receptor, Cripto.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TMEFF1
• Uniprot:
  • Q6PFE7()