TM4SF4 Antibody (832441) [Allophycocyanin]

HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human TM4SF4 APC-conjugated Monoclonal Antibody (Catalog # FAB7998A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 832441
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

TM4SF4 Antibody (832441) [Allophycocyanin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human TM4SF4
  Accession # P48230
• Specificity: Detects human TM4SF4 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TM4SF4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TM4SF4 Antibody (832441) [Allophycocyanin]

• FLJ31015
• ILTMP
• il-TMP
• ILTMPintestinal and liver (il) tetraspan membrane protein
• Intestine and liver tetraspan membrane protein
• TM4SF4
• transmembrane 4 L six family member 4
• transmembrane 4 L6 family member 4
• transmembrane 4 superfamily member 4

Background

TM4SF4 (Transmembrane 4 L6 Family Member 4), also known as IL-TMP (Intestine and Liver Tetraspanin Membrane Protein), is a member of the L6 tetraspanin family of molecules. It is expressed at highest levels on non-proliferating villus-associated intestinal epithelia and periportal hepatocytes, with increased expression in hepatocellular carcinomas. It is thought to mediate proliferation and adhesion. Its molecular size is reported at approximately 21 kDa for the unglycosylated form and 25-40 kDa for various glycosylated forms.  The 202 amino acid (aa) human TM4SF4 contains four transmembrane domains and two potential N-glycosylation sites. It shares 88% aa sequence identity with mouse and rat TM4SF4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TM4SF4 Antibody (FAB7998A)(1)

Publication using FAB7998A

• Natalie DeForest, Babu Kavitha, Siqi Hu, Roi Isaac, Lynne Krohn, Minxian Wang et al. Human gain-of-function variants in HNF1A confer protection from diabetes but independently increase hepatic secretion of atherogenic lipoproteins Cell Genomics 2023-07-12 [PMID: 37492105]

Bioinformatics

• Gene Symbol: TM4SF4
• Uniprot:
  • P48230()