TIP120A Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TIP120A Antibody Summary

Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 11-24 of Human CAND1/TIP120A (N-terminal) coupled to KLH.
Specificity
This product reacts with human, rat and mouse CAND1/TIP120A. Cross reactivity does occur with human, rat and mouse CAND2/TIP120B.
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Gene
CAND1
Purity
Sterile filtered
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
The antibody immunoprecipitates in vitro translated protein and protein from cell lysates. Coimmunoprecipitation of related proteins has not been tested. A 136.4 kDa band corresponding - human CAND1 is detected. Most cell lines expressing CAND1 can be used as a positive control. Researchers should determine optimal titers for other applications.
Publications
Read Publications using NBP1-78047.

Reactivity Notes

Cross reactivity with TIP120A from other sources has not been tested.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Antiserum
Preservative
0.01% Sodium Azide
Purity
Sterile filtered

Alternate Names for TIP120A Antibody

  • cullin-associated and neddylation-dissociated 1
  • Cullin-associated and neddylation-dissociated protein 1
  • cullin-associated NEDD8-dissociated protein 1
  • DKFZp434M1414
  • FLJ90441
  • KIAA0829FLJ10114
  • p120 CAND1
  • TBP interacting protein
  • TBP-interacting protein 120A
  • TBP-interacting protein of 120 kDa A
  • TIP120AFLJ38691
  • TIP120FLJ10929

Background

CAND1 is also known as TIP120A, and TATA-binding protein-interacting protein 120A. The SCF complex consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, and functions as an E3 ubiquitin ligase. E3 ubiquitin ligases regulate various physiological processes. CAND1 binds to Cul1 and potentially regulates the SCF complex. CAND1 physically associates with Cul1 in the nucleus and this interaction is mediated by a central region of Cul1 distinct from its binding sites for Skp1 and Rbx1. CAND1 selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation. CAND1 forms a ternary complex with CUL1 and ROC1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIP120A Antibody (NBP1-78047)(3)

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Video Protocols

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Bioinformatics

Gene Symbol CAND1
Uniprot