TIMP-1 Antibody [Alexa Fluor® 405]

• Reactivity: Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

TIMP-1 Antibody [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat TIMP-1
  Cys24-Ala217
  Accession # P30120
• Specificity: Detects rat TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) TIMP‑1 and recombinant mouse TIMP-1 is observed and less than 1% cross-reactivity with rhTIMP-2, rh
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIMP-1 Antibody [Alexa Fluor® 405]

• CLGI
• Collagenase inhibitor
• collagenase inhibitor)
• EPATIMP-1
• EPO
• erythroid potentiating activity
• Erythroid-potentiating activity
• Fibroblast collagenase inhibitor
• FLJ90373
• HCI
• metalloproteinase inhibitor 1
• TIMP metallopeptidase inhibitor 1
• TIMP1
• TIMP-1
• TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity
• Tissue inhibitor of metalloproteinases 1

Background

Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family: TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 32‑34 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.