Thyroglobulin Antibody (rTGB/4744)

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Immunohistochemistry-Paraffin: Thyroglobulin Antibody (rTGB/4744) [NBP3-07351] - Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Thyroglobulin Mouse Recombinant Monoclonal Antibody (rTGB/4744).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow, IHC-P
Clone
rTGB/4744
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Thyroglobulin Antibody (rTGB/4744) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Human thyroid follicular cells
Localization
Cytoplasmic and secreted
Specificity
Reacts with a partially defined epitope of human thyroglobulin. This epitope is different form the epitope recognized by antibody clone 6E1. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TG
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08264)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Thyroglobulin Antibody (rTGB/4744)

  • AITD3
  • AITD3TGN
  • cog
  • TDH3
  • Tg
  • TGN
  • Thyroglobulin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Blogs on Thyroglobulin.

TTF1 / NKX2.1 - An essential regulator of lung development with implications in cancer diagnostics
Thyroid transcription factor 1 (TTF-1), also known as NKX2.1, is a conserved master regulatory transcription factor involved in the development of the lung, brain, and thyroid (1). In the lung TTF-1 positively regulates the expression of several lu...  Read full blog post.

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Bioinformatics

Gene Symbol TG