Recombinant Human Thymosin beta 4 Protein

SDS-Page: Human Thymosin beta 4 Protein [NBP2-35087]

• Reactivity: Hu
• Applications: Func, PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human Thymosin beta 4 Protein Summary

• Description: A single, non-glycosylated biologically active polypeptide chain corresponding to 43 residues of TMSB4X.
  
  

  Source: E. coli

  Amino Acid Sequence:
  SDKPDMAEIE KFDKSKLKKT ETQEKNPLPS KETIEQEKQA GES

• Preparation Method: Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
• Details of Functionality: Thymosin beta 4 protein is fully biologically active when compared to standard. The biological activity determined by its ability to produce a protective effect against hydrogen peroxide in primary lung fibroblasts is in a concentration range of 0.5 - 10 ug/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TMSB4X
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

• Theoretical MW: 4.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human Thymosin beta 4 Protein

• PTMB4
• T beta-4
• TB4
• TB4X
• TB4Xbeta 4, X chromosome
• THYB4
• Thymosin beta 4
• thymosin beta 4, X-linked
• thymosin beta-4
• TMSB4
• TMSB4X

Background

Thymosin Beta 4 is a naturally occurring peptide encoded by the TMSB4X gene located on Chr. X in humans. It is found in high concentrations in blood platelets, wound fluid and other tissues in the body. T beta-4 is a major actin regulating peptide and the primary function is to stimulate the productions of T cells, which plays important part of the immune system. The thymosin beta- 4 peptide, if used after a heart attack, might reactivate cardiac progenitor cells to repair damaged heart tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TMSB4X
• Uniprot:
  • P62328()