Recombinant Human Thymosin beta 4 Protein

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SDS-Page: Human Thymosin beta 4 Protein [NBP2-35087]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

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Catalog# & Formulation Size Price

Recombinant Human Thymosin beta 4 Protein Summary

Description
A single, non-glycosylated biologically active polypeptide chain corresponding to 43 residues of TMSB4X.

Source: E. coli

Amino Acid Sequence:
SDKPDMAEIE KFDKSKLKKT ETQEKNPLPS KETIEQEKQA GES

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
Thymosin beta 4 protein is fully biologically active when compared to standard. The biological activity determined by its ability to produce a protective effect against hydrogen peroxide in primary lung fibroblasts is in a concentration range of 0.5 - 10 ug/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
TMSB4X
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
4.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human Thymosin beta 4 Protein

  • PTMB4
  • T beta-4
  • TB4
  • TB4X
  • TB4Xbeta 4, X chromosome
  • THYB4
  • Thymosin beta 4
  • thymosin beta 4, X-linked
  • thymosin beta-4
  • TMSB4
  • TMSB4X

Background

Thymosin Beta 4 is a naturally occurring peptide encoded by the TMSB4X gene located on Chr. X in humans. It is found in high concentrations in blood platelets, wound fluid and other tissues in the body. T beta-4 is a major actin regulating peptide and the primary function is to stimulate the productions of T cells, which plays important part of the immune system. The thymosin beta- 4 peptide, if used after a heart attack, might reactivate cardiac progenitor cells to repair damaged heart tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol TMSB4X
Uniprot