Recombinant Human THTPA Protein

Images

 
Western Blot: THTPA Protein [NBP1-45325] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1.0 mg/ml

Order Details

Recombinant Human THTPA Protein Summary

Description
A recombinant protein with a His tag and corresponding to amino acids 1 - 230 of THTPA.

Source: E. coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAQGLIEVER KFLPGPGTEE RLQELGGTLE YRVTFRDTYY DTPELSLMQA DHWLRRREDS GWELKCPGAA GVLGPHTEYK ELTAEPTIVA QLCKVLRADG LGAGDVAAVL GPLGLQEVAS FVTKRSAWKL VLLGADEEEP QLRVDLDTAD FGYAVGEVEA LVHEEAEVPT ALEKIHRLSS MLGVPAQETA PAKLIVYLQR FRPQDYQRLL EVNSSRERPQ ETEDPDHCLG

Preparation
Method
E.coli
Protein/Peptide Type
Recombinant Protein
Gene
THTPA
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
The purity of this protein is > 905 by SDS - PAGE. Molecular weight is 27.7 kDa (250aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher) The His tag increases overall size to 250 residues.
Theoretical MW
27.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This is a Human protein

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT
Concentration
1.0 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human THTPA Protein

  • EC 3.6.1.28
  • MGC2652
  • thiamine triphosphatase
  • thiamine-triphosphatase
  • THTP
  • THTPASE

Background

THTPA, also known as THTP or THTPASE, is a member of the THTPase family. This protein is localized to the cytoplasm and expressed at low levels in a variety of tissues, including testis, uterus, prostate, bladder, lung and kidney. THTPA is a hydrolase that catalyzes the H2O-dependent hydrolysis of thiamine triphosphate (THTP) to thiamine diphosphate (THDP), the major form of thiamine within the cell. THTPA exists as a monomer and functions at an optimal pH of 8.5. Recombinant human THTPA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol THTPA

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