Thioredoxin Reductase 1/TRXR1 Antibody (JA11-32)

Western Blot: Thioredoxin Reductase 1/TRXR1 Antibody (JA11-32) [NBP2-67194] - Analysis of TXNRD1 on Hela (1) and human liver tissue lysate (2) using anti-TXNRD1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Thioredoxin Reductase 1/TRXR1 Antibody (JA11-32) [NBP2-67194] - Staining TXNRD1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Thioredoxin Reductase 1/TRXR1 Antibody (JA11-32) [NBP2-67194] - Analysis of paraffin-embedded mouse testes tissue using anti-TXNRD1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Thioredoxin Reductase 1/TRXR1 Antibody (JA11-32) [NBP2-67194] - Flow cytometric analysis of SH-SY5Y cells with Thioredoxin Reductase 1/TRXR1 antibody at 1/100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: Thioredoxin Reductase 1/TRXR1 Antibody (JA11-32) [NBP2-67194] - Analysis of paraffin-embedded human kidney tissue using anti-TXNRD1 antibody. Counter stained with hematoxylin.

Product Discontinued

Thioredoxin Reductase 1/TRXR1 Antibody (JA11-32) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein with Human Thioredoxin Reductase 1/TRXR1 aa 1-649 / 649. (SwissProt: Q16881 Human; SwissProt: Q9JMH6 Mouse; SwissProt: O89049 Rat)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TXNRD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Thioredoxin Reductase 1/TRXR1 Antibody (JA11-32)

• EC 1.8.1
• EC 1.8.1.9
• Gene associated with retinoic and IFN-induced mortality 12 protein
• Gene associated with retinoic and interferon-induced mortality 12 protein
• GRIM12
• GRIM-12
• GRIM-12TR1
• KDRF
• KM-102-derived reductase-like factor
• MGC9145
• oxidoreductase
• Thioredoxin Reductase 1
• thioredoxin reductase 1, cytoplasmic
• thioredoxin reductase GRIM-12
• Thioredoxin reductase TR1
• TR
• TR1
• TRXR1
• TRXRD1
• TXNR
• TXNRD1
• TXNRTRXR1

Background

thioredoxin reductase 1 is a pyridine nucleotide oxidoreductase which reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. It is thought to function as a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine residue which is required for the catalytic activity. Alternative splicing results in several transcript variants encoding the same protein. Additional transcript variants have been described but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TXNRD1