Thioredoxin Reductase 1/TRXR1 Antibody (489804) [Alexa Fluor™ Plus 405]

• Reactivity: Hu, Mu, Rt
• Clone: 489804
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 405

Thioredoxin Reductase 1/TRXR1 Antibody (489804) [Alexa Fluor™ Plus 405] Summary

• Specificity: Detects mouse Thioredoxin Reductase 1/TRXR1 in direct ELISAs and human, mouse, and rat Thioredoxin Reductase 1/TRXR1 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thioredoxin Reductase 1/TRXR1 Antibody (489804) [Alexa Fluor™ Plus 405]

• EC 1.8.1
• EC 1.8.1.9
• Gene associated with retinoic and IFN-induced mortality 12 protein
• Gene associated with retinoic and interferon-induced mortality 12 protein
• GRIM12
• GRIM-12
• GRIM-12TR1
• KDRF
• KM-102-derived reductase-like factor
• MGC9145
• oxidoreductase
• Thioredoxin Reductase 1
• thioredoxin reductase 1, cytoplasmic
• thioredoxin reductase GRIM-12
• Thioredoxin reductase TR1
• TR
• TR1
• TRXR1
• TRXRD1
• TXNR
• TXNRD1
• TXNRTRXR1

Background

Thioredoxin reductase 1 (TRXR1) is an approximately 70 kDa member of the class-I pyridine nucleotide-disulfide oxidoreductase family.  Human TRXR1 is 649 amino acids (aa) in length. Residues 151-152 constitute a propeptide that is deleted from the mature protein. Splicing variants produce five additional isoforms for human TRXR1. Isoform 2 has a 32 aa substitution for aa 107-138 and a deletion of aa 1-106. Isoform 3 has a deletion of aa 1-51 and a 49 aa substitution for aa 52-100. Isoform 4 is missing aa 1-98 and has a 3 aa substitution for aa 99-101. Isoform 5 has a deletion of aa 1-150. Residues 56-156 make up a glutaredoxin domain, and residues 520-632 constitute a pyridine nucleotide-disulphide oxidoreductase dimerization domain. In addition, there are three phosphotyrosines at positions 161, 163, and 281, and a selenocysteine at position 648. Human TRXR1 shares 74% and 70% aa sequence identity with mouse and rat TRXR1, respectively. Isoform 1 is involved in glutaredoxin activity as well as thioredoxin reductase activity, and it induces actin and tubulin polymerization, which leads to formation of cell membrane protrusions.  Isoform 4 has been shown to enhance the transcriptional activity of the beta receptor only. Finally, isoform 5 mediates cell death induced by a combination of interferon-beta and retinoic acid. Isoform 1 is expressed mostly in the Leydig cells, but also in the ovary, spleen, heart, liver, kidney, and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in the kidney, uterus, testis, ovary, prostate, placenta, and fetal liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.