Thioredoxin-like 5/TRP14/TXNDC17 Antibody (544710)

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat acute T cell leukemia cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Thioredoxin-like 5/TRP14 Monoclonal Antibody (Catalog # MAB3504) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin-like 5/TRP14 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu
• Applications: WB
• Clone: 544710
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Thioredoxin-like 5/TRP14/TXNDC17 Antibody (544710) Summary

• Immunogen: E. coli-derived recombinant human TRP14
  Ala2-Asp123
  Accession # Q9BRA2
• Specificity: Detects endogenous human TRP14 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TXNDC17
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thioredoxin-like 5/TRP14/TXNDC17 Antibody (544710)

• MGC14353
• Protein 42-9-9
• thioredoxin (Trx)-related protein, 14 kDa
• thioredoxin domain containing 17
• thioredoxin domain-containing protein 17
• Thioredoxin like 5
• Thioredoxin-like 5
• Thioredoxin-like protein 5
• TRP14
• TRP1414 kDa thioredoxin-related protein
• TXNDC17
• TXNL5

Background

Thioredoxin-like 5, also known as a 14 kDa thioredoxin-related protein (TRP14) is a cytosolic redox regulatory molecule that belongs to the thioredoxin family of proteins. It contains a thioredoxin-like redox-sensitive active site motif (CPDC). In its reduced form, the active site cysteine residues reduce protein disulfide. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by the NADPH-dependent thioredoxin reductase 1. Thioredoxin-like 5 is ubiquitously expressed and acts on protein targets different from that of Thioredoxin-1. The amino acid sequence of human Thioredoxin-like 5 is 80% identical to that of mouse and rat Thioredoxin-like 5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thioredoxin-like 5/TRP14/TXNDC17 Antibody (MAB3504)(1)

Publication using MAB3504

• Éva Dóka, Irina Pader, Adrienn Bíró, Katarina Johansson, Qing Cheng, Krisztina Ballagó et al. A novel persulfide detection method reveals protein persulfide- and polysulfide-reducing functions of thioredoxin and glutathione systems Science Advances 2016-01-22 [PMID: 26844296]

Bioinformatics

• Gene Symbol: TXNDC17
• Uniprot:
  • Q9BRA2()