TfR (Transferrin R) Antibody [Unconjugated]

Western blot shows lysates of ZR-75 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human TfR (Transferrin R) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2474) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TfR (Transferrin R) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TfR (Transferrin R) was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human TfR (Transferrin R) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2474) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Effect of Pcsk9 deletion on LDLR and CD81 levels in age- and sex-matched Pcsk9-/- mice (n = 5) and their wild type littermates (n = 4).Shown are (A) mean ± SE serum LDL-C levels and (B) Western blot analysis of CD81 and LDLR levels in liver extracts with TfR as loading control, in animals sacrificed after a 4-hour fast. Lanes in panel B represent samples from individual mice. LDL-C, low-density lipoprotein cholesterol; LDLR, low-density lipoprotein receptor; PCSK9, proprotein convertase subtilisin/kexin type 9; SE, standard error; TfR, transferrin receptor; wt, wild type. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0154498), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TfR (Transferrin R) Antibody [Unconjugated] Summary

• Immunogen: Human placenta-derived TfR (Transferrin R)
• Specificity: Detects human TfR (Transferrin R) in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TFRC
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TfR (Transferrin R) Antibody [Unconjugated]

• CD71 antigen
• CD71
• CD71TRFR
• p90
• T9
• TfR (Transferrin R)
• TfR (TransferrinR)
• TfR
• TFR1
• TFRC
• TR
• transferrin receptor (p90, CD71)
• transferrin receptor protein 1
• TRFR

Background

The Transferrin Receptor (TfR or TfR-1, designated CD71) is a type 2 transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a 188 kDa disulfide-linked homodimer of 95 kDa monomers (1-4). As the major mediator of cellular iron uptake, it binds and internalizes diferric transferrin, allowing iron release at the low pH of the endosome (2, 5). The human TfR cDNA encodes 760 amino acids (aa) including a 67 aa N-terminal intracellular domain, a 21 aa transmembrane domain, and a 672 aa extracellular domain (ECD) with helical, peptidase (nonfunctional), and ligand binding domains, including an RGD potential integrin binding site (5). Human TfR ECD shares 75 - 80% aa identity with mouse, rat, feline, canine, equine, porcine and bovine TfR. A 679 aa alternately spliced form begins at aa 82 and is presumably secreted, while in an 804 aa form, 44 aa are inserted at aa 518 within the peptidase region (6). Most soluble TfR (sTfR) arises from trypsin proteolysis at aa 100, producing the circulating form of TfR (3). sTfR concentration in plasma or serum is proportional to total TfR and can be increased by iron deficiency (3). Erythroid progenitors, which use iron for hemoglobin synthesis, normally account for the bulk of total body TfR production (3). Since rapidly growing cells require iron to replicate DNA, cancer cells can express up to 5-fold more TfR than quiescent cells in the surrounding tissue (2, 4). Antibody targeting of TfR can inhibit tumor cell proliferation and induce apoptosis (2, 4). The hereditary hemochromatosis protein HFE competes with diferric transferrin for binding to TfR, and targets TfR for degradation rather than recycling (2, 5). TfR has been reported to have ferritin-independent functions in T cell development, immunological synapse formation and galectin-3-mediated cell death, and to be a cell entry receptor for New World hemorrhagic fever arenaviruses (2, 4, 7).

1. Schneider, C. et al. (1984) Nature 311:675.
2. Daniels, T.R. et al. (2006) Clin. Immunol. 121:144.
3. Skikne, B.S. (2008) Am. J. Hematol. 83:872.
4. Macedo, M.F. and M. deSousa (2008) Inflamm. Allergy Drug Targets 7:41.
5. Aisen, P. (2004) Int. J. Biochem. Cell Biol. 36:2137.
6. Entrez protein Accession # EAW53671, EAW53672.
7. Radoshitzky, S.R. et al. (2007) Nature 446:92.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TfR (Transferrin R) Antibody (AF2474)(13)

Publications using AF2474

• DK Bailey, W Clark, DJ Kosman The iron chelator, PBT434, modulates transcellular iron trafficking in brain microvascular endothelial cells PLoS ONE, 2021-07-26;16(7):e0254794. 2021-07-26 [PMID: 34310628] (Western Blot, Human)
• R Yamamoto, E Yoden, N Tanaka, M Kinoshita, A Imakiire, T Hirato, K Minami Nonclinical safety evaluation of pabinafusp alfa, an anti-human transferrin receptor antibody and iduronate-2-sulfatase fusion protein, for the treatment of neuronopathic mucopolysaccharidosis type II Molecular genetics and metabolism reports, 2021-04-18;27(0):100758. 2021-04-18 [PMID: 33981582] (Surface Plasmon Resonance, Human)
• JW Rhee, H Yi, D Thomas, CK Lam, N Belbachir, L Tian, X Qin, J Malisa, E Lau, DT Paik, Y Kim, BS Choi, N Sayed, K Sallam, R Liao, JC Wu Modeling Secondary Iron Overload Cardiomyopathy with Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cell Rep, 2020-07-14;32(2):107886. 7/14/2020 [PMID: 32668256]
• J Hu, W Wang, C Liu, M Li, E Nice, H Xu Receptor tyrosine kinase inhibitor Sunitinib and integrin antagonist peptide HM-3 show similar lipid raft dependent biphasic regulation of tumor angiogenesis and metastasis J. Exp. Clin. Cancer Res., 2019-08-28;38(1):381. 2019-08-28 [PMID: 31462260] (Western Blot, Human)
• IF Scheiber, NO Alarcon, N Zhao Manganese Uptake by A549 Cells is Mediated by Both ZIP8 and ZIP14 Nutrients, 2019-06-28;11(7):. 2019-06-28 [PMID: 31261654] (Western Blot, Human)
• JS Spence, R He, HH Hoffmann, T Das, E Thinon, CM Rice, T Peng, K Chandran, HC Hang IFITM3 directly engages and shuttles incoming virus particles to lysosomes Nat. Chem. Biol., 2019-01-14;0(0):. 2019-01-14 [PMID: 30643282] (Western Blot, Human)
• Elise F. Villard, Aurélie Thedrez, Jorg Blankenstein, Mikaël Croyal, Thi-Thu-Trang Tran, Bruno Poirier et al. PCSK9 Modulates the Secretion But Not the Cellular Uptake of Lipoprotein(a) Ex Vivo: An Effect Blunted by Alirocumab JACC: Basic to Translational Science 10/31/2016 [PMID: 29308438]
• Zhenyu Cai, Anling Zhang, Swati Choksi, Weihua Li, Tao Li, Xue-Min Zhang et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration Cell Research 8/1/2016 [PMID: 27444869]
• Jiali Cai, Bingxin Gu, Fengwen Cao, Shiyuan Liu A transferrin-target magnetic/fluorescent dual-mode probe significantly enhances the diagnosis of non-small cell lung cancer Oncotarget 6/28/2016 [PMID: 27223075]
• Alirocumab, a Therapeutic Human Antibody to PCSK9, Does Not Affect CD81 Levels or Hepatitis C Virus Entry and Replication into Hepatocytes PLoS ONE, 2016-04-26;11(4):e0154498. 2016-04-26 [PMID: 27115873] (Western Blot, Mouse)
• Changyi Ji, Daniel J. Kosman Molecular mechanisms of non-transferrin-bound and transferring-bound iron uptake in primary hippocampal neurons Journal of Neurochemistry 6/1/2015 [PMID: 25649872]
• Staquicini FI, Ozawa MG, Moya CA, Driessen WH, Barbu EM, Nishimori H, Soghomonyan S, Flores LG, Liang X, Paolillo V, Alauddin MM, Basilion JP, Furnari FB, Bogler O, Lang FF, Aldape KD, Fuller GN, Höök M, Gelovani JG, Sidman RL, Cavenee WK, Pasqualini R, Arap W Systemic combinatorial peptide selection yields a non-canonical iron-mimicry mechanism for targeting tumors in a mouse model of human glioblastoma. J. Clin. Invest., 2010-12-22;121(1):161-73. 2010-12-22 [PMID: 21183793] (IHC-Fr, Human)
• Harris B, Pereira I, Parkin E Targeting ADAM10 to lipid rafts in neuroblastoma SH-SY5Y cells impairs amyloidogenic processing of the amyloid precursor protein. Brain Res., 2009-08-11;1296(0):203-15. 2009-08-11 [PMID: 19679113] (Western Blot, Human)

Review for TfR (Transferrin R) Antibody (AF2474) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Teddy Chan WB Human 02/08/2019

Summary

• Application: Western Blot
• Sample Tested: Brain Vascular Smooth Muscle Cells
• Species: Human
• Lot: USI011805A

Bioinformatics

• Gene Symbol: TFRC