Recombinant Human TFIIB His Protein

SDS-Page: Recombinant Human TFIIB Protein [NBP1-30313] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: .25 mg/ml

Recombinant Human TFIIB His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-316 of Human TFIIB
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK YQNRRTMSSS DRAMMNAFKE ITTMADRINL PRNIVDRTNN LFKQVYEQKS LKGRANDAIA SACLYIACRQ EGVPRTFKEI CAVSRISKKE IGRCFKLILK ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV ELDLVPGRSP ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF PTDFKFDTPV DKLPQL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GTF2B
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 36.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM MES buffer (pH6.0), 30% glycerol, 0.1 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: .25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human TFIIB His Protein

• general transcription factor IIB
• General transcription factor TFIIB
• S300-II
• TFIIBTF2BRNA polymerase II transcription factor IIB
• transcription initiation factor IIB

Background

GTF2B, also known as transcription factor II B, is one of several general transcription factors that make up the RNA polymerase II preinitiation complex. It localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. The protein serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. It is involved in the selection of the transcription start site. Recombinant GTF2B protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for TFIIB Recombinant Protein (NBP1-30313)(1)

Publication using NBP1-30313

• Palacio M, Taatjes D Real-time visualization of reconstituted transcription reveals RNAPII activation mechanisms at single promoters Cell reports 2025-09-23 [PMID: 40914943]

Bioinformatics

• Gene Symbol: GTF2B