TFEC Antibody

Western Blot: TFEC Antibody [NB100-1031] - (1ug/ml) of Human Testis lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TFEC Antibody Summary

• Description: Novus Biologicals Goat TFEC Antibody (NB100-1031) is a polyclonal antibody validated for use in WB and ELISA. Anti-TFEC Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence TLDHQIINPTLK-C corresponding to N-Terminus according to NP_036384.1, NP_001018068.1.
• Specificity: This antibody is expected to recognise both reported Human isoforms according to NP_036384.1 and NP_001018068.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TFEC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 40-45 kDa band observed in human testis lysate.
• Control:

  Human Testis Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TFEC Antibody

• Class E basic helix-loop-helix protein 34
• hTFEC-L
• TCFECTFECLbHLHe34BHLHE34
• TFE-C
• transcription factor EC
• Transcription factor EC-like

Background

Transcriptional regulator that acts as a repressor or an activator. Acts as a transcriptional repressor on minimal promoter containing element F (that includes an E-box sequence). Binds to element F in an E-box sequence-specific manner. Acts as a transcriptional transactivator on the proximal promoter region of the tartrate-resistant acid phosphatase (TRAP) E-box containing promoter . Collaborates with MITF in target gene activation . Acts as a transcriptional repressor on minimal promoter containing mu E3 enhancer sequence . Binds to mu E3 DNA sequence of the immunoglobulin heavy-chain gene enhancer . Binds DNA in a homo- or heterodimeric form

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TFEC Antibody (NB100-1031)(3)

Publications using NB100-1031

• Jankowska M, Rogalska J, Wyszkowska J, Stankiewicz M. Molecular Targets for Components of Essential Oils in the Insect Nervous System—A Review Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry 2017-12-23 [PMID: 29295521]
• Musk GC, James A, Kemp MW et al. Pregnant Sheep in a Farm Environment Did Not Develop Anaemia Animals : an Open Access Journal from MDPI 2017-04-25 [PMID: 28441332]
• Yasumoto K, Shibahara S. Molecular cloning of cDNA encoding a human TFEC isoform, a newly identified transcriptional regulator. Biochim Biophys Acta 1997-07-17 [PMID: 9256061]

Bioinformatics

• Gene Symbol: TFEC
• Entrez:
  • 22797(Human)
• Uniprot:
  • NP_036384(Human)