TFEB Antibody (CL14112) - Azide and BSA Free

Analysis in human tonsil and liver tissues using NBP3-44287 antibody. Corresponding TFEB RNA-seq data are presented for the same tissues.

Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.

Staining of human liver shows very weak positivity in hepatocytes.

Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Staining of human tonsil shows strong nuclear and cytoplasmic positivity in non-germinal center cells.

Staining of human small intestine shows strong nuclear positivity in glandular cells.

• Reactivity: Hu
• Applications: IHC
• Clone: CL14112
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TFEB Antibody (CL14112) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TFEB Antibody (CL14112) - Azide and BSA Free (NBP3-26853) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P19484, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TFEB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for TFEB Antibody (CL14112) - Azide and BSA Free

• ALPHATFEB
• BHLHE35
• Class E basic helix-loop-helix protein 35
• T-cell transcription factor EB
• TCFEB
• TCFEBbHLHe35
• TFEB
• transcription factor EB

Background

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TFEB