TFEB Antibody (CL14112) - BSA Free

Staining of human small intestine shows strong nuclear positivity in glandular cells.

Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.

Analysis in human tonsil and liver tissues. Corresponding RNA-seq data are presented for the same tissues.

Staining of human liver shows very weak positivity in hepatocytes.

Staining of human tonsil shows strong nuclear and cytoplasmic positivity in non-germinal center cells.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: CL14112
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TFEB Antibody (CL14112) - BSA Free Summary

• Description: Novus Biologicals Mouse TFEB Antibody (CL14112) - BSA Free (NBP3-26853) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from P19484, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TFEB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TFEB Antibody (CL14112) - BSA Free

• ALPHATFEB
• BHLHE35
• Class E basic helix-loop-helix protein 35
• T-cell transcription factor EB
• TCFEB
• TCFEBbHLHe35
• TFEB
• transcription factor EB

Background

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TFEB