Testican 3/SPOCK3 Antibody [Biotin]

• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Testican 3/SPOCK3 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Testican 3/SPOCK3
  Ala26-Ile436
  Accession # Q8BKV0
• Specificity: Detects mouse Testican 3/SPOCK3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human (rh) Testiscan 1 and rhTestican2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SPOCK3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Mouse Testican 3/SPOCK3 Antibody (Catalog # MAB2346)
  Standard: Recombinant Mouse Testican 3/SPOCK3 (Catalog # 2346-PI)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Testican 3 encoded by the SPOCK3 gene is a proteoglycan expressed in brain (1). The human and mouse cDNAs predict 90% identity between the deduced amino acid sequences from the two species, indicating a conserved function (2, 3). Only the human protein, but not the mouse protein, has been characterized in the literature. Testican 3 contains Ca²⁺-binding domain and the C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. The N-terminal region, which is unique to testicans, is responsible for the inhibition of Testican 3 towards MMP-14 (MT1-MMP, a metalloprotease) activation of MMP-2 (1). The thyropin domain and the follistatin-like domain with a six cysteine Kazal-like motif may inhibit cysteine and serine proteases, respectively (4). A spliced variant designated as N-Tes contains the N-terminal unique region, the follistatin-like domain and the Ca²⁺-binding domain, but lacks the C-terminal thyropin domain and the acidic domain (1). The purified rmTestican 3 is capable of inhibiting rhMMP-14 and rhCathepsin L (R&D Systems, Catalog # 918-MP and 952-CY) in assays using the fluorogenic peptide substrates (R&D Systems, Catalog # ES001 and ES008). As compared to rhTestican 1 (R&D Systems, Catalog # 2327-PI), the IC₅₀ of rmTestican 3 is weaker toward rhCathepsin L and rhMMP-14 activity.

1. Nakada, M. et al. (2001) Cancer Res. 61:8896.
2. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
3. Okazaki, Y. et al. (2002) Nature 420:563.
4. Alliel, P.M. et al. (1993) Eur. J. Biochem. 214:347.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPOCK3