Testican 2/SPOCK2 Antibody (327403) [Alexa Fluor™ Plus 555]

• Reactivity: Hu
• Clone: 327403
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 555

Testican 2/SPOCK2 Antibody (327403) [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects human Testican 2/SPOCK2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Testican 1 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Testican 2/SPOCK2 Antibody (327403) [Alexa Fluor™ Plus 555]

• FLJ97039
• KIAA0275
• KIAA0275CWCV, and Kazal-like domains proteoglycan 2
• sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2
• SPOCK2
• Testican 2

Background

Testican 2 encoded by the SPOCK2 gene is a proteoglycan highly expressed in brain (1, 2). The human and mouse cDNAs predict 94% identity between the deduced amino acid sequences from the two species, indicating a conserved function (3, 4). Testican 2 contains a Ca²⁺-binding domain and a C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. However, these domains may not be involved directly in the inhibition of targeted proteases. For example, Testican 2 is not able to inhibit MMP-14 (MT1-MMP, a metalloprotease) activation of MMP-2 (5). Nonetheless, overexpression of Testican 2 is able to abrogate inhibition of MMP-14 by other testican family members and to inhibit neurite outgrowth (6, 7).

1. Nagase, T. et al. (1996) DNA Res. 3:321.
2. Vannahme, C. et al. (1999) J. Neurochem. 73:12.
3. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
4. Okazaki, Y. et al. (2002) Nature 420:563.
5. Nakada, M. et al. (2001) Cancer Res. 61:8896.
6. Nakada, M. et al. (2003) Cancer Res. 63:3364.
7. Hartmann, U. and P. Maurer (2001) Matrix Biol. 20:23.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.