TERT Antibody

Product Discontinued

TERT Antibody Summary

• Immunogen: Synthetic peptide corresponding to amino acids 1104-1123 of hTERT.
• Marker: Embryonic Stem Cell Marker
• Specificity: The antibody detects ectopically-expressed hTERT and high levels of endogenous hTERT, but several non-specific bands appear on immunoblots as well. Although it has been reported that this antibody reacts with mouse TERT (mTERT) (see Drissi, et al. 2001), the binding to mTERT is considerably weaker and less specific than the binding to hTERT (not shown).
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TERT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:50000
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2ul per mg of Lysate
  • Western Blot 1:500-1:2000
• Application Notes: In immunofluorescence microscopy assays, staining with anti-TERT-16 was specific to the nuclei of cells with ectopic TERT expression.
• Theoretical MW: 127 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross-reacts with Human. Weak cross-reactivity with Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TERT Antibody

• EST2Telomerase-associated protein 2
• hEST2
• hTERT
• TCS1EC 2.7.7.49
• Telomerase catalytic subunit
• telomerase reverse transcriptase
• TERT
• TP2HEST2
• TRTEC 2.7.7

Background

Telomerase is a reverse transcriptase that adds telomeric repeats (TTAGGG)n to chromosomal ends, compensating for the telomere shortening that occurs with DNA replication. In normal human somatic cells, telomerase is repressed and telomeres progressively shorten, leading to limited lifespan and senescence. Reactivation of telomerase activity is associated with human cancer and cell immortalization. Approximately 85% of human cancers, including breast, prostate, stomach, bladder, colon, and liver cancer, have telomerase activity, whereas most normal somatic cells do not. The specificity of telomerase to human cancer has led to investigations of telomerase activity and expression as a tumor marker. For example, the presence of telomerase activity in human urine has been identified as a marker for human bladder carcinoma. Human telomerase consists of three major subunits: a catalytic protein subunit called hTERT (for human TElomerase Reverse Transcriptase), a template RNA called hTR, and telomerase-associated protein (TEP-1). TERT and hTR are minimally required to reconstitute telomerase activity in vitro. In human cells, hTR is constitutively expressed; TERT transcription is a primary mechanism for regulation of telomerase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TERT Antibody (NB600-476)(3)

Publications using NB600-476

• Tang H, Ge L, Shao W et al. Effect of targeted silencing of hTERT mRNA by lentivirus-mediated siRNA on A549 lung cancer cells in vitro Mol Biol Rep 2012-10-09 [PMID: 23054018] (WB, Human)
• Ahmed S, JF Passos, MJ Birket, T Beckmann, S Brings, H Peters, MA Birch-Machin, T von Zglinicki, G Saretzki. Telomerase does not counteract telomere shortening but protects mitochondrial function under oxidative stress. J Cell Sci;121(Pt 7):1046-53. 2008-04-01 [PMID: 18334557]
• Wu YL, Dudognon C, Nguyen E et al. Immunodetection of human telomerase reverse-transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths. J Cell Sci 2006-07-01 [PMID: 16772337]

Bioinformatics

• Gene Symbol: TERT
• Entrez:
  • 7015(Human)
• Uniprot:
  • O14746()