TERT Antibody (2D8) - BSA Free

Western Blot: TERT Antibody (2D8) [NB100-297] - Analysis of human TERT, using NB100-297. Sample: MJ90 whole cell lysate.

Immunocytochemistry/Immunofluorescence: TERT Antibody (2D8) [NB100-297] - Detection of Tert (green) in HepG2 cells using NB100-297. Nuclei (Blue) are counterstained with Hoechst 33258.

Immunohistochemistry-Paraffin: TERT Antibody (2D8) [NB100-297] - TERT was detected in immersion fixed paraffin-embedded sections of human liver cancer using anti-human mouse monoclonal antibody (Catalog # NB100-297) at 1:600 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, KD
• Clone: 2D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

TERT Antibody (2D8) - BSA Free Summary

• Immunogen: Full-length recombinant human Telomerase reverse transcriptase from insect cells. [UniProt# O14746]
• Localization: Nuclear
• Marker: Embryonic Stem Cell Marker
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: TERT
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Frozen 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Knockdown Validated
  • Western Blot 1:500-1:1000
• Application Notes: In Western blot, this antibody recognizes a band at ~127 kDa and additional non-specific background band ~110 kDa may also be seen. Note that the isotype is IgM and the appropriate secondary should be used. For immunopurification of telomerase, please see the procedure guide for a detailed protocol.
• Theoretical MW: 127 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for TERT Antibody (2D8) - BSA Free

• EST2Telomerase-associated protein 2
• hEST2
• hTERT
• TCS1EC 2.7.7.49
• Telomerase catalytic subunit
• telomerase reverse transcriptase
• TERT
• TP2HEST2
• TRTEC 2.7.7

Background

Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication that is active in progenitor as well as cancer cells, and stays inactive or show very low activity in normal somatic cells. TERT is the catalytic component of the holoenzyme complex (TERT, DKC1, WDR79/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) that implicates in telomeres elongation by acting as a reverse transcriptase adding simple sequence repeats to chromosome ends by copying a template sequence within RNA component of the enzyme. For executing its versatile functions, TERT can interact with HSP90A , PTGES3, HSPA1A, RAN , XPO1, PTPN11, NCL, SMARCA4, MCRS1, PIF1, PML and GNL3L. TERT modulates Wnt signaling and plays important roles in aging and anti-apoptosis. Telomerase activation has been implicated in cell immortalization/ carcinogenesis and defects in TERT are associated with susceptibilty to aplastic anemia, coronary artery disease (CAD), dyskeratosis congenita autosomal dominant type 2 (DKCA2), pulmonary fibrosis, and/or bone marrow failure, telomere-related, type 1 (PFBMFT1), dyskeratosis congenita autosomal recessive type 4 (DKCB4), susceptibility to pulmonary fibrosis idiopathic (IPF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TERT Antibody (NB100-297)(9)

Publications using NB100-297

• Akiyama M, Ozaki K, Kawano T et al. Telomerase activation as a repair response to radiation-induced DNA damage in Y79 retinoblastoma cells. Cancer Lett 2013-07-11 [PMID: 23850566] (WB, Human)
• Heeg S, Hirt N, Queisser A et al. EGFR overexpression induces activation of telomerase via PI3K/AKT-mediated phosphorylation and transcriptional regulation through Hif1-alpha in a cellular model of oral-esophageal carcinogenesis Cancer Sci 2011-02-01 [PMID: 21156006] (WB, Human)
• Fabricius EM, Kruse-Boitschenko U, Khoury R et al. Localization of telomerase hTERT protein in frozen sections of basal cell carcinomas (BCC) and tumor margin tissues. Int J Oncol. 2009-12-01 [PMID: 19885561] (IHC-Fr, Human)
• Fabricius EM, Kruse-Boitschenko U, Khoury R et al. Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues. Int J Oncol 2009-05-01 [PMID: 19360339] (IF/IHC, Human)
• Xiao Y, Gao X, Gannot G et al. Quantitation of HER2 and telomerase biomarkers in solid tumors with IgY antibodies and nanocrystal detection. Int J Cancer;122(10):2178-86. 2008-05-15 [PMID: 18214859]
• Zhang JG, Eguchi J, Kruse CA et al. Antigenic profiling of glioma cells to generate allogeneic vaccines or dendritic cell-based therapeutics. Clin Cancer Res;13(2 Pt 1):566-75. 2007-01-15 [PMID: 17255279] (ICC/IF, FLOW, Human)
• Deissler H, Deissler H, Lang GK et al. Generation and characterization of iBREC: novel hTERT-immortalized bovine retinal endothelial cells. Int J Mol Med. 2005-07-01 [PMID: 15942679] (WB, Human)
• Masutomi, K et al. The telomerase reverse transcriptase regulates chromatin state DNA damage responses. PNAS 102(23): 8222-8227. 2005-01-01 [PMID: 15928077] (WB, Human)
• Blasco MA, Hahn WC. Evolving views of telomerase and cancer. Trends Cell Biol;13(6):289-94. 2003-06-01 [PMID: 12791294]

Bioinformatics

• Gene Symbol: TERT
• Entrez:
  • 7015(Human)
• Uniprot:
  • O14746()